长期冷冻对ELISA法检测血浆标本中HBsAg结果的影响  

Effect of long-term frozen plasma on the detection of HBsAg by ELISA

作　　者：连斯敏 陈晓华 林树波 郭自约 肖泽斌 LIAN Simin;CHEN Xiaohua;LIN Shubo;GUO Ziyue;XIAO Zebin(Shantou Blood Station,Shantou 515064,China)

机构地区：[1]汕头市中心血站,广东汕头515064

出　　处：《中国输血杂志》2024年第1期91-94,共4页Chinese Journal of Blood Transfusion

摘　　要：目的评价献血者HBsAg阳性血液标本在-20°C冻存8年后ELISA法HBsAg检测的结果,评估血站目前留样保存方式的有效性。方法收集本站2014年5月—2015年3月100份经HBsAg ELISA检测阳性的献血者血浆标本,冻存在-20°C冰箱,于2023年解冻标本并通过同种方法再次检测。结果100份血浆标本的HBsAg再检定性结果均为阳性,再检符合率100%,冻存后S/CO值降低明显(27.52 vs 19.03,P<0.05)。结论长期冻存会使HBsAg ELISA检测S/CO值下降,但不影响阳性定性结果。Objective To evaluate the HBsAg detection results of HBsAg+samples after 8 years refrigeration by ELISA and evaluate the effectiveness of the current storage policy of retained samples.Methods A total of 100 HBsAg+plasma samples by ELISA from May 2014 to March 2015 and refrigerated at-20℃were collected and retested for HBsAg using the same method after thawing in 2023.Results The HBsAg retest results of 100 plasma samples were all positive,with the concordance rate of 100%,though there was a significant decrease in the S/CO value after refrigeration(27.52 vs 19.03,P<0.05).Conclusion Long-term refrigeration can lead to a decrease in the S/CO value of HBsAg ELISA detection,but it does not affect the positive results.

关 键 词：HBsAg S/CO值 冻存血浆标本 献血者 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学] R373.2