抗-PP1P^(k)的血清学特性探究及P血型相关文献分析  

Serological characteristics of anti-PP1P^(k)and literature review on P blood group system

作　　者：李金龙 叶水文[2] 孙君 陈亚南 向东[4] LI Jinlong;YE Shuiwen;SUN Jun;CHEN Yanan;XIANG Dong(Xingtai People’s Hospital,Xingtai 054000,China;The Second Affiliated Hospital of Nanchang University;Zibo First Hospital;Shanghai Blood Center)

机构地区：[1]邢台市人民医院,河北邢台054000 [2]南昌大学第二附属医院 [3]淄博市第一医院 [4]上海市血液中心

出　　处：《中国输血杂志》2024年第1期101-106,共6页Chinese Journal of Blood Transfusion

摘　　要：目的本研究旨在深入了解抗-PP1P^(k)的血清学特性,探索小p血型孕妇顽固性流产的可能性治疗方法。方法通过使用鸽子蛋清、人血浆中和法;2-巯基乙醇试剂破坏IgM抗体;添加补体成分等方法对抗-PP1P^(k)进行探究。在不同处理条件、不同介质、不同温度下测定抗-PP1P^(k)的效价,研究人血清对抗-PP1P^(k)及其致敏补体能力的中和作用。结果所采用抗-PP1P^(k)在盐水和柱凝集中的效价分别为4和8,低温会使抗-PP1P^(k)效价增高,2-巯基乙醇破坏会明显降低其效价。鸽子蛋清可以部分中和抗-PP1P^(k)。人血浆同样可以降低抗-PP1P^(k)的效价,其中和能力高于鸽子蛋清,且中和能力存在个体差异。结论抗-PP1P^(k)是1种IgM和IgG并存、冷性、可以激活补体的混合抗体。人血浆可以有效降低抗-PP1P^(k)效价以及激活补体的能力,输注中和能力高的血浆有望成为治疗p表型孕妇习惯性流产的新方法。Objective To comprehensively explore the serological characteristics of anti-PP1P^(k)and potential therapeutic strategies for recurrent miscarriage in p-blood type pregnant women.Methods Neutralization with pigeon egg white and human plasma,disruption of IgM antibodies by 2-mercaptoethanol reagent,and complement adding were conducted.Anti-PP1P^(k)titers under different processing conditions,media and temperatures were determined,and neutralizing effect of human plasma on anti-PP1P^(k)and its sensitization complement ability were analyzed.Results The titers of anti-PP1P^(k)in saline and column agglutination were 4 and 8,respectively.Low temperature increased titers,whileβ-mercaptoethanol treatment significantly reduced them.Pigeon egg white partially neutralized anti-PP1P^(k)antibodies.Human plasma was also capable of reducing anti-PP1P^(k)titers with neutralization capability surpassing that of pigeon egg white,and there were individual differences in neutralization capability.Conclusion The anti-PP1P^(k)was a blend of antibodies encompassing both IgM and IgG types,exhibiting cold reactivity,and having the potential for complement activation.Human plasma emerges as an effective modulator for diminishing the efficacy of anti-PP1P^(k).Plasma transfusion holds promise as a therapeutic avenue for addressing recurrent miscarriages in pregnant individuals with the p phenotype.

关 键 词：抗-PP1P^(k) P1P^(k)血型系统 Globoside血型系统 鸽子蛋清 中和实验 

分 类 号：R457.11[医药卫生—治疗学]