一种基于三苯胺-噻吩基团的新型荧光探针在藻细胞中检测CN‒的应用  

A Novel Triphenylamine-thiophene Dyad Fluorescent Probe for Cyanide Detection in Living Algae Cells

作　　者：文丹[1] 喻艳华[1] WEN Dan;YU Yanhua(Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education,School of Optoelectronic Materials&Technology,Jianghan University,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学光电化学材料与器件教育部重点实验室,光电材料与技术学院,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2024年第1期5-15,共11页Journal of Jianghan University：Natural Science Edition

基　　金：Jianghan University Science and Technology Special Project(2011KJZX001)。

摘　　要：氰根离子(CN‒)是最具毒性的阴离子之一,一些食用藻类可以从其生活环境中释放氰化物或吸收氰化物,这可能对人类健康造成危害。因此,设计并合成了一种新的荧光探针TI,可用于在活体藻类细胞中检测CN‒。探针TI由三苯胺-噻吩部分和吲哚部分组成,其分子结构中碳氮双键易与CN‒发生亲核加成反应,碳氮双键中共轭π键被打断,探针TI在473 nm处出现显著的蓝色荧光,从而可用来检测CN‒。探针TI可单一性识别CN‒,最低检测限可达95.7 nmol/L。最重要的是,探针TI可用于实时检测活藻类细胞和食用藻类中的CN‒。Cyanide(CN‒)is one of the most well-known toxic anions,some edible algae can release cyanide or absorb cyanide from their living environments,which may cause harm to human health.Therefore,we designed and synthesized a novel fluorescent probe TI for CN‒detection in living algae cells.Probe TI was constructed by triphenylamine-thiophene moiety and indolium moiety and exhibited remarkable blue fluorescence at 473 nm upon treatment with cyanide due to the nucleophilic attack of CN‒on the C=N bond.Moreover,probe TI demonstrated excellent selectivity and sensitivity(LOD 95.7 nmol/L),and a fast responsive time for cyanide detection in an aqueous solution.Most importantly,probe TI could be applied for real-time detection of CN‒in living algae cells and edible algae.

关 键 词：三苯胺-噻吩 吲哚 CN‒检测 荧光探针 藻细胞 

分 类 号：O657.39[理学—分析化学]