推拿促进房水内源性前列腺素的生成调节大鼠睫状体淋巴管增生  

Massage Regulates Lymphatic Hyperplasia in Ciliary Body of Rats by Promoting Generation of Aqueous Humor Prostaglandin

作　　者：池华博文 李青松[1,2] 贾琳琳 符之瑄 许愿[1] 杨倩 杨俊倚 CHI Huabowen;LI Qingsong;JIA Linlin;FU Zhixuan;XU Yuan;YANG Qian;YANG Junyi(Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China;Putuo Hospital Affiliated to Clinical Medical School of Anhui Medical University,Shanghai 200062,China)

机构地区：[1]上海中医药大学附属普陀医院,上海200062 [2]安徽医科大学附属普陀临床医学院,上海200062

出　　处：《世界中医药》2023年第22期3187-3193,3201,共8页World Chinese Medicine

基　　金：国家自然科学基金项目(82074495);上海市医学重点专科-眼科建设项目(A-263);上海市普陀区卫生自主创新科研基金项目(17-PT-07);上海市普陀区自主创新(重大)项目(15-PT-01);2020年卫健委临床研究课题(202040148)。

摘　　要：目的:探讨推拿促进大鼠眼内前列腺素的生成,诱导大鼠眼部睫状体内淋巴管的增生,进而降低青光眼眼压的作用机制。方法:将48只SD大鼠造模后随机分为青光眼模型组、推拿组、拉坦前列腺素组和推拿结合拉坦前列腺素组,每组12例。治疗过程中分别抽取各组不同时间段房水进行酶联免疫吸附试验(ELISA),采用蛋白质印迹法、定量聚合酶链反应、免疫荧光法、免疫组织化学法检测同源盒基因转录因子1(Prox-1)、5′-核苷酸酶(5′-Nase)表达水平,淋巴管酶组织化学染色法检测各组睫状体内淋巴管的增生情况。结果:推拿结合拉坦前列腺素组眼压均低于其他组(P<0.05);推拿结合拉坦前列腺素组中Prox-1、5′-Nase表达水平均高于其他组(P<0.05);推拿结合拉坦前列腺素组睫状体组织淋巴增殖均高于其他组。结论:推拿可以有效促进睫状体淋巴管的增生,而增生的淋巴管能吸收多余的房水导致眼压下降。Objective:To explore the mechanism of massage in reducing intraocular pressure in glaucoma by promoting the generation of aqueous humor prostaglandin and inducing the intraocular lymphatic hyperplasia in rats.Methods:Forty-eight SD rats were randomized into glaucoma model,massage,latanoprost,and massage+latanoprost groups(n=12).The aqueous humor samples were collected from each group at different time points during the treatment period for enzyme-linked immunosorbent assay.Western blotting,qPCR,immunofluorescence assay,and immunohistochemical staining were employed to determine the expression levels of prospero homeobox 1(Prox-1) and 5′-Nase.Histochemical staining was employ to detect the lymphatic hyperplasia in the ciliary body in each group.Results:The massage+latanoprost group had lower intraocular pressure(P<0.05),higher expression levels of Prox-1 and 5′-Nase(P<0.05),and higher rate of lymphatic hyperplasia in the ciliary body than other groups.Conclusion:Massage can promote the lymphatic hyperplasia in the ciliary body,which can facilitate the absorption of excess aqueous humor to reduce intraocular pressure.

关 键 词：推拿 房水 前列腺素 拉坦前列腺素 眼压 睫状体 淋巴管 青光眼 

分 类 号：R244.1[医药卫生—针灸推拿学] R276.7[医药卫生—中医临床基础] R775[医药卫生—中医学]