大理地区杂合型片形吸虫幼虫期越冬的实验观察  

Experimental observation on overwintering development of hybrid Fasciola fluke miracidiae in Dali

作　　者：陈凤 赵银娇 张冬琦 郝明明 李诗韵 张剑平 周岩 许学年 陈绍荣 刘榆华 罗天鹏 CHEN Feng;ZHAO Yinjiao;ZHANG Dongqi;HAO Mingming;LI Shiyun;ZHANG Jianping;ZHOU Yan;XU Xuenian;CHEN Shaorong;LIU Yuhua;LUO Tianpeng(Dali Autonomous Prefecture Institute of Schistosomiasis Control and Prevention,Dali,Yunnan 671000,China;National Institute of Parasitic Diseases,Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research),WHO Collaborating Centre for Tropical Diseases,National Center for International Research on Tropical Diseases,National Health Commission Key Laboratory of Parasite and Vector Biology,Shanghai 200025,China.)

机构地区：[1]大理白族自治州血吸虫病防治研究所,云南大理671000 [2]中国疾病预防控制中心寄生虫病预防控制所(国家热带病研究中心)、WHO热带病合作中心、国家热带病国际联合研究中心、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室,上海200025

出　　处：《海峡预防医学杂志》2023年第4期1-5,共5页Strait Journal of Preventive Medicine

基　　金：云南省高层次卫生健康技术人才培养专项经费资助(媒介生物控制学H-2018072);国家寄生虫资源库资助(NPRC-2019-194-30)。

摘　　要：目的 研究大理地区冬春季室温条件下杂合型片形吸虫毛蚴孵化及其在中间宿主小土蜗体内发育过程,为该虫种防制提供依据。方法 收集大理地区片形吸虫成虫虫卵进行孵化,逐日观察孵化情况,并于冬春季节人工感染小土蜗、室内饲养,观察感染及各期幼虫发育情况。结果 第一批虫卵孵化及感染:2017年12月25日起,平均室温15.5℃,毛蚴59 d孵出;2018年2月22日至4月16日,平均室温20℃情况下,毛蚴感染小土蜗53 d发现母雷蚴、新生及未成熟的子雷蚴。第二批虫卵孵化及感染:2018年10月26日起,平均室温17℃情况下,毛蚴30 d孵出;2018年11月25日至2019年3月1日感染小土蜗饲养,平均室温15℃,95 d左右可成功结囊。感染小土蜗的分子鉴定结果显示,实验虫种为杂合型片形吸虫。结论 在大理地区室内平均温度15℃以上的情况下,杂合型片形吸虫毛蚴仍可越冬发育到感染阶段。Objective To study the hatching and development process of hybrid Fasciola miracidiae in the intermediate host,Galba pervia,under ambient temperature conditions in winter and spring seasons in Dali,Yunnan Province.Results of this study can provide a scientific basis for fasciolosis control and prevention.Methods Fasciola eggs were collected for hatching from adult flukes in Dali.Daily monitoring was conducted to observe the hatching process.Artificial infection with Fasciola was carried out for Galba pervia during winter and spring seasons,which were then raised indoors.The progression of infection and the development of larval in all stages were closely monitored.Results First batch of eggs\hatching and infection:Hatching of egg was starting on December 25,2017,at an average room temperature of 15.5℃,and miracidiae was successfully hatched on day 59;Between February 22 and April 16,2018,at an average room temperature of 20℃,in Galba pervia infected with miracidiae,we observed mother rediae,newly born rediae,and immature daughter rediae on day 53.Second batch of eggs\hatching and infection:Hatching of egg was starting on October 26,2018,at an average room temperature of 17℃,and miracidiae was successfully hatched on day 30;Between November 25,2018 and March 1,2019,infected Galba pervia were grown indoors at an average room temperature of 15℃.Successful encystment metacercariae was observed on day 95.Results of molecular identification showed that the parasite species under experiment belong to hybrid Fasciola flukes.Conclusion In Dali,hybrid Fasciola fluke miracidiae can overwinter and develop into infection stage under indoor conditions with an average temperature exceeding 15℃.

关 键 词：杂合型片形吸虫 小土蜗 中间宿主 越冬发育 感染实验 

分 类 号：R383.2[医药卫生—医学寄生虫学]