Bo′s abdominal acupuncture improves disordered metabolism in obese type 2 diabetic rats through regulating fibroblast growth factor 21 and its related adipokines  

作　　者：QIN Xihui PANG Jianli XIONG Guan FENG Jie 

机构地区：[1]School of Pharmaceutical Sciences,Guangxi Medical University,Nanning 530021,China [2]Department of Endocrinology,Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,China [3]Department of Gynecology and obstetrics,the Eighth People’s Hospital of Nanning,Nanning 530007,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第6期1200-1208,共9页中医杂志（英文版）

基　　金：the National Natural Science Foundation of China (81560731),to Explore the Mechanism of the Regulation of Bo’s Abdominal Acupuncture on Obesity Type 2 Diabetes based on fibroblast growth factor 21 Signaling Pathway

摘　　要：OBJECTIVE:To investigate the effect of Bo′s abdominal acupuncture(BOAA)on fibroblast growth factor 21(FGF21)and its related adipokines in type 2 diabetes mellitus(T2DM)rats.METHODS:This study established obese T2DM rat model by high-fat diet(HFD)with a dose of streptozotocin(STZ,30 mg/kg).Obese T2DM rats were randomly subdivided into four groups(n=10):negative,BOAA,conventional acupuncture(COA group)and metformin group(Met group)groups.The biochemical parameters,mRNAs,and proteins were analyzed using enzyme-lined immunoassays kits,quantitative polymerase chain reaction and Western blot.RESULTS:Treatment with BOAA attenuated the histopathological changes in visceral fat and restored the alterations in the levels of body weight,fasting blood glucose(FBG),homeostasis model assessment for insulin resistance(HOMA-IR).BOAA treatment significantly decreased the levels of triglyceride,total cholesterol,low density lipoprotein cholesterol,leptin,and increased the serum levels of high-density lipoprotein cholesterol,fibroblast growth factor 21(FGF21),adiponectin(ADP),peroxisome proliferator-activated receptorγ(PPAR-γ),C-peptide(C-P)in obese T2DM rats.Furthermore,BOAA treatment significantly increased the mRNA expressions of FGF21,ADP,leptin,PPAR-γ,PPAR-αand adenosine 5‘-monophosphate(AMP)-activated protein kinase(AMPK).Besides,BOAA treatment upregulated the protein expressions of fibroblast growth factor receptors3(FGFR3),PPAR-α,extracellular signal-regulated kinase(ERK),phosphorylated ERK(p-ERK),AMPK,p-AMPK,Liver kinase B1(LKB1),phosphorylated LKB1(p-LKB1),acetyl-CoA carboxylase(ACC)and phosphorylated ACC(p-ACC),while downregulated the protein expressions of FGF21 and PPAR-γin visceral fat.CONCLUSIONS:BOAA treatment reduced FBG and body weight,and improved insulin sensitivity through regulating FGF21 signaling pathway and its related adipokine in obese T2DM rats.

关 键 词：diabetes mellitus type 2 obesity fibroblast growth factors ADIPOKINES insulin resistance Bo′s abdominal acupuncture 

分 类 号：R245[医药卫生—针灸推拿学]