球孢白僵菌Bb378菌株的分离、鉴定及对草地贪夜蛾的致病性研究  

Isolation and identification of the Beauveria bassiana Bb378 strain and its pathogenicity to Spodoptera furgiperda

作　　者：毕思佳 吴晨源 胡本进 徐丽娜 徐婷婷 胡飞 BI Si-Jia;WU Chen-Yuan;HU Ben-Jin;XU Li-Na;XU Ting-Ting;HU Fei(Institute of Plant Protection and Agro-products Safety,Anhui Academy of Agricultural Sciences,Hefei 230031,China)

机构地区：[1]安徽省农业科学院植物保护与农产品质量安全研究所,合肥230031

出　　处：《应用昆虫学报》2023年第4期1223-1232,共10页Chinese Journal of Applied Entomology

基　　金：国家重点研发计划项目(2021YFD1400700);安徽省自然科学基金(2008085MC75)。

摘　　要：【目的】筛选出对草地贪夜蛾Spodoptera frugiperda高致病性的球孢白僵菌Bb378,并进一步研究菌株对玉米生长的影响及对草地贪夜蛾的驱避效果,为应用球孢白僵菌防治草地贪夜蛾奠定基础。【方法】分别用0.05%Tween-80水溶液和1×10^(4)、1×10^(5)、1×10^(6)、1×10^(7)和1×10^(8)孢子/m L的球孢白僵菌孢子悬浮液接种草地贪夜蛾3龄幼虫,第3、7及14天后观察记录感染情况及死亡虫数;分别用浸种法、灌根法、叶面喷施法研究球孢白僵菌Bb378菌株对玉米的促生性及对草地贪夜蛾取食行为的影响。【结果】筛选出的高致病性菌株Bb378处理草地贪夜蛾3龄幼虫14 d后累计校正死亡率为48.61%,LC50为1.11×10^(8)孢子/m L。使用叶面喷施法接种,球孢白僵菌Bb378对玉米叶面积和鲜重的影响最大;使用灌根法和浸种法接种,Bb378菌株对株高的影响较为明显;当Bb378浓度为1×10^(7)和1×10^(8)孢子/m L时对玉米的促生长效果较为显著。浓度为1×10^(7)和1×10^(8)孢子/m L悬浮液浸种对初孵和3龄幼虫均具有显著的驱避效果;1×10^(7)和1×10^(8)孢子/m L悬浮液灌根对初孵幼虫驱避效果明显,但对3龄幼虫仅在1×10^(8)孢子/m L浓度下有明显作用;叶面喷施法与对照差异不显著。【结论】球孢白僵菌Bb378对草地贪夜蛾具有较高致病性,接种后促进了玉米生长,形成的球孢白僵菌Bb378-玉米共生体可抑制草地贪夜蛾的取食行为,具有进一步研究和开发的价值。[Objectives]To investigate the potential of using the fungus Beauveria bassiana to protect maize crops from Spodoptera frugiperda,a polyphagous,important,invasive,insect pest,that poses a major threat to agriculture in China.[Methods]The Beauveria bassiana Bb378 strain,which is highly pathogenic to S.frugiperda,was isolated and 3rd instar S.frugiperda larvae were inoculated with a 0.05%Tween-80 aqueous solution containing either 1×10^(4),1×10^(5),1×10^(6),1×10^(7),or 1×10^(8)conidia/mL of B.bassiana spore suspension.The infection rate and number of dead larvae were observed and recorded after 3,7 and 14 days.Effects of the Bb378 strain on the feeding of S.frugiperda on maize were studied by using the seed soaking,root irrigation and foliar spraying methods.[Results]After 14 days the cumulative corrected mortality and LC50 of the highly pathogenic Bb378strain were 48.61%and 1.11×10^(8)conidia/mL,respectively.Spraying the Bb378 strain onto maize foliage increased the leaf area and fresh weight of corn,whereas root irrigation and seed soaking had an obvious effect on plant height.The growth promoting effect of the Bb378 strain was obvious at concentrations of 1×10^(7)and 1×10^(8)conidia/mL.Soaking seeds in suspensions of 1×10^(7)and 1×10^(8)conidia/mL significantly repelled newly hatched and 3rd instar larvae.Irrigating roots with suspensions of 1×10^(7)and 1×10^(8)conidia/mL also had an obvious repellent effect on newly hatched larvae,but only a concentration of 1×10^(8)conidia/mL significantly repelled 3rd instar larvae.There was,however,no significant difference between leaf spraying and the control.[Conclusion]The Bb378 strain is highly pathogenic to S.frugiperda.Applying B.bassiana to maize not only promoted plant growth,but also inhibited the feeding behavior of S.frugiperda,findings that justify further research and development.

关 键 词：草地贪夜蛾 球孢白僵菌Bb378 杀虫活性 促生性 驱避作用 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治] S433.4[农业科学—植物保护]