异位表达赤霉素降解酶基因促进木本植物小桐子成花转变  

Ectopic Expression of Gibberellin-degrading Gene Promoted Floral Transition in Woody Plant Jatropha curcas

作　　者：胡颖雄 明新 陶彦彬 徐增富[2,3] Hu Yingxiong;Ming Xin;Tao Yanbin;Xu Zengfu(Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403;Key Laboratory of Tropical Plant Resources and Sustainable Use,Innovative Academy of Seed Design,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Mengla,666303;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Department of Forestry,Guangxi University,Nanning,530004)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]中国科学院西双版纳热带植物园,种子创新研究院,热带植物资源可持续利用重点实验室,勐腊666303 [3]广西大学林学院,亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《分子植物育种》2024年第2期439-445,共7页Molecular Plant Breeding

基　　金：国家自然科学基金面上项目(31370595);中国科学院项目(kfj-brsn-2018-6-008;XTBG-T02)共同资助。

摘　　要：小桐子(Jatropha curcas L.)是一种多年生木本油料作物,其营养生长过于旺盛是其种子产量低的原因之一。赤霉素在大多数多年生木本植物中抑制成花转变。为了降低小桐子内源赤霉素含量、促进植株的生殖生长,本研究采用拟南芥花特异性表达的AtAP1(Arabidopsis thaliana APETALA1)启动子驱动小桐子赤霉素降解酶基因JcGA2ox6在小桐子中表达。结果显示:转基因小桐子的叶片颜色变深,叶面积变小,单株总花序数目显著增多,花序变小而紧凑,且雌花和雄花变小,雌雄花数目均减少,单个花序中的雌花比例降低。本研究结果表明通过特异性表达小桐子JcGA2ox6能促进其由营养生长向生殖生长的转变。Physic nut(Jatropha curcas L.)is a perennial woody oil plant.Its vegetative growth is excessive,which is one of the factors restricting its seed yield.Gibberellin(GA)inhibits floral formation in most perennial woody plants.Shoot reproductive growth could be promoted by reducing endogenous gibberellin content.Therefore,in this study,a Jatropha gibberellin 2-oxidase 6(JcGA2ox6)which catalyzes GA degradation was driven by a flower-specific promoter,Arabidopsis thaliana APETALA1(AtAP1)promoter,in Jatropha.The results showed that the leaves became darker and smaller,the numbers of inflorescence per plant were significantly increased,the inflorescences became smaller and compact,both female and male flowers decreased in size and number,and the ratios of female to male flowers decreased in AtAP1:JcGA2ox6 transgenic Jatropha.Our results showed that flower-specific expressions of JcGA2ox6 could promote the transition from vegetative to reproductive growth in Jatropha.

关 键 词：小桐子 赤霉素 JcGA2ox6 开花 多年生 

分 类 号：S565.9[农业科学—作物学]