m^(6)A Profile Dynamics Indicates Regulation of Oyster Development by m^(6)A-RNA Epitranscriptomes  

作　　者：Lorane Le Franc Bruno Petton Pascal Favrel Guillaume Rivière 

机构地区：[1]Laboratoire de Biologie des Organismes et des Ecosyste`mes Aquatiques(BOREA),Muséum d’Histoire Naturelle,Sorbonne Université,Universitéde Caen Normandie,Universitédes Antilles,CNRS UMR 8067,IRD,14032 Caen,France [2]Ifremer,Laboratoire des Sciences de l’Environnement Marin,UMR 6539 CNRS/UBO/IRD/Ifremer,Centre Bretagne,29280 Plouzané,France

出　　处：《Genomics, Proteomics & Bioinformatics》2023年第4期742-755,共14页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the French national program CNRS EC2CO ECODYN(Ecosphère Continentale et Cótière’HERITAGe’;Grant No.2019-200919362L)awarded to Guillaume Rivière and the Council of the Normandy Region(RIN ECUME;Grant No.18E01643-18P02383)awarded to Pascal Favrel.

摘　　要：The N6-methylation of RNA adenosines(N6-methyladenosine,m^(6)A)is an important regulator of gene expression with critical implications in vertebrate and insect development.However,the developmental significance of epitranscriptomes in lophotrochozoan organisms remains unknown.Using methylated RNA immunoprecipitation sequencing(MeRIP-seq),we generated transcriptome-wide m^(6)A-RNA methylomes covering the entire development of the oyster from oocytes to juveniles.Oyster RNA classes display specific m^(6)A signatures,with messenger RNAs(mRNAs)and long non-coding RNAs(lncRNAs)exhibiting distinct profiles and being highly methylated compared to transposable element(TE)transcripts.Epitranscriptomes are dynamic and correspond to the chronological steps of development(cleavage,gastrulation,organogenesis,and metamorphosis),with minimal mRNA and lncRNA methylation at the morula stage followed by a global increase.mRNA m^(6)A levels are correlated with transcript levels,and shifts in methylation profiles correspond to expression kinetics.Differentially methylated transcripts cluster according to embryo-larval stages and bear the corresponding developmental functions(cell division,signal transduction,morphogenesis,and cell differentiation).The m^(6)A level of TE transcripts is also regulated and peaks during the gastrulation.We demonstrate that m^(6)A-RNA methylomes are dynamic and associated with gene expression regulation during oyster development.The putative epitranscriptome implication in the cleavage,maternal-to-zygotic transition,and cell differentiation in a lophotrochozoan model brings new insights into the control and evolution of developmental processes.

关 键 词：RNA methylation Transcription EMBRYO METAMORPHOSIS EVO-DEVO 

分 类 号：Q42[生物学—神经生物学]