基于UPLC-Q-Exactive MS技术3种核桃类种仁中甘油酯组成比较  

Comparison on glycerolipid composition in three kinds of walnut kernels based on UPLC-Q-Exactive MS technology

作　　者：谭文玥 许梦洋 柳成航 王紫嫣 郭忠仁[2,3,4] 贾晓东 TAN Wenyue;XU Mengyang;LIU Chenghang;WANG Ziyan;GUO Zhongren;JIA Xiaodong(Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen),Nanjing 210014,China;State Key Laboratory of Desert and Oasis Ecology,Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830011,China;Yili Botanical Garden,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Yili 835800,China〕)

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏省中国科学院植物研究所(南京中山植物园),江苏省植物资源研究与利用重点实验室,江苏南京210014 [3]中国科学院新疆生态与地理研究所,荒漠与绿洲生态国家重点实验室,干旱区生态安全与可持续发展重点实验室,新疆乌鲁木齐830011 [4]中国科学院新疆生态与地理研究所伊犁植物园,新疆伊犁835800

出　　处：《植物资源与环境学报》2024年第1期87-97,共11页Journal of Plant Resources and Environment

基　　金：江苏省林业科技创新与推广项目(LYKJ-句容[2020]01);江苏省科学技术厅重点研发计划项目(BE2021349)。

摘　　要：基于超高效液相色谱-四级杆-轨道阱高分辨质谱联用(UPLC-Q-Exactive MS)技术比较分析了山核桃(Carya cathayensis Sarg.)、胡桃(Juglans regia Linn.)和薄壳山核桃〔Carya illinoinensis(Wangenh.)K.Koch〕3种木本油料作物种仁中甘油酯的组成及差异,结合LipidSearch软件和混合脂质内标对脂质分子进行定性及定量分析。结果显示:3种核桃类种仁中均鉴定到298个甘油酯,包括245个甘油三酯、38个甘油二酯、1个单半乳糖甘油二酯、9个双半乳糖甘油二酯和5个硫代异鼠李糖甘油二酯。偏最小二乘法判别分析得到104个变量投影重要度(VIP)大于1的脂质分子。山核桃和薄壳山核桃种仁中甘油三酯的主要脂肪酸为油酸,胡桃种仁中甘油三酯的主要脂肪酸为亚油酸。3种核桃类种仁中甘油二酯的主要脂肪酸均为亚油酸,其次为油酸。山核桃和薄壳山核桃种仁的甘油酯组成更为相近。UPLC-Q-Exactive MS可作为鉴别这3种木本油料作物种仁中甘油酯组成的有效方式。The composition and differences of glycerolipids in kernels of three woody oil crops,namely Carya cathayensis Sarg.,Juglans regia Linn.,and Carya illinoinensis(Wangenh.)K.Koch were compared and analyzed based on ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry(UPLC-Q-Exactive MS)technology,and qualitative and quantitative analyses were conducted for lipid molecules in combination with LipidSearch software and mixed lipid internal standard.The results show that a total of 298 glycerolipids are identified in three kinds of walnut kernels,containing 245 triacylglycerols,38 diacylglycerols,1 monogalactosyl diacylglycerol,9 digalactosyl diacylglycerols,and 5 sulfoquinovosyl diacylglycerols.One hundred and four lipid molecules with variable important in projection(VIP)greater than 1 are obtained via partial least squares-discriminant analysis.The main fatty acid of triacylglycerols in kernels of C.cathayensis and C.illinoinensis is oleic acid,while that in kernels of J.regia is linoleic acid.The main fatty acid of diacylglycerols in three kinds of walnut kernels are all linoleic acid,followed by oleic acid.The composition of glycerolipids in kernels of C.cathayensis and C.illinoinensis are closer.UPLC-Q-Exactive MS can be used as an effective method to identify the glycerolipid composition in the kernels of these three woody oil crops.

关 键 词：山核桃 胡桃 薄壳山核桃 甘油酯 脂肪酸 UPLC-Q-Exactive MS 

分 类 号：S664.1[农业科学—果树学] TQ646[农业科学—园艺学]