基于UPLC-ESI-MS/MS技术黑豆种皮的化学成分分析  

Analysis on chemical components in seed coat of black soybean based on UPLC-ESI-MS / MS technology

作　　者：吴志华 马丽[2] 陈剑[2] 周佳宇 WU Zhihua;MA Li;CHEN Jian;ZHOU Jiayu(Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Key Laboratory for the Research and Utilization of Plant Resources,Jiangsu Provincial Service Center for Anti-diabetic Drugs Screening,Institute of Botany,Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen),Nanjing 210014,China〕)

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏省中国科学院植物研究所(南京中山植物园),江苏省植物资源研究与利用重点实验室江苏省抗糖尿病药物筛选技术服务中心,江苏南京210014

出　　处：《植物资源与环境学报》2024年第1期111-113,共3页Journal of Plant Resources and Environment

基　　金：江苏省植物资源研究与利用重点实验室开放基金项目(JSPKLB202201)。

摘　　要：采用超高效液相色谱-电喷雾-串联质谱(UPLC-ESI-MS/MS)技术对黑豆种皮体积分数70%乙醇提取物中的化合物进行分析。结果表明:黑豆种皮体积分数70%乙醇提取物中32个化合物主要为多酚类,其中,酚酸类化合物的相对含量为13.38%,黄酮类化合物的相对含量为39.59%。儿茶素的相对含量最高(10.15%),反式阿魏酸的相对含量次之(7.86%),二氢黄豆苷元和原花青素B 1的相对含量也较高(分别为5.52%和5.36%)。黑豆种皮中主要的多酚类物质为酚酸类和黄酮类化合物,具有进一步开发利用的价值。The compounds in the volume fraction of 70%ethanol extracts from seed coat of black soybean were analyzed by using ultra-high performance liquid chromatography-electrospray-tandem mass spectrometry(UPLC-ESI-MS/MS)technology.The results show that 32 compounds in the volume fraction of 70%ethanol extracts from seed coat of black soybean are mainly polyphenols,in which,the relative content of phenolic acids is 13.38%,and that of flavonoids is 39.59%.The relative content of catechin is the highest(10.15%),followed by trans-ferulic acid(7.86%),and the contents of dihydrodaidzein and procyanidin B_(1) are also relatively high(which are 5.52%and 5.36%respectively).The main polyphenols in seed coat of black soybean are phenolic acids and flavonoids,which have valuable potential for further development and utilization.

关 键 词：黑豆种皮 化学成分 UPLC-ESI-MS/MS 

分 类 号：Q946.8[生物学—植物学] S567.219[农业科学—中草药栽培] R284.2[农业科学—作物学]