乳腺癌miR-4500、NGF表达与术前磁共振征象的相关性研究  

Correlation analysis of miR-4500,NGF expressions with preoperative magnetic resonance signs in breast cancer

作　　者：杨帆 陈静[1] 张斌[1] 李娇娇[1] 张丽 边桂风 边雅静[1] 郎晓燕[1] YANG Fan;CHEN Jing;ZHENG Bin;LI Jiao-jiao;ZHANG Li;BIAN Gui-feng;BIAN Ya-jing;LANG Xiao-yan(Department of Medical Imaging,First Affiliated Hospital of Hebei North University,Zhangjiakou Hebei 075000,China)

机构地区：[1]河北北方学院附属第一医院医学影像部,河北张家口075000

出　　处：《局解手术学杂志》2024年第1期47-50,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：河北省2021年度医学科学研究课题计划(20210136)。

摘　　要：目的分析乳腺癌患者中miR-4500、神经生长因子(NGF)表达与术前磁共振(MR)征象的相关性。方法选取我院收治的乳腺癌患者105例,通过qRT-PCR法检测患者乳腺癌组织及癌旁组织中miR-4500、NGF mRNA表达水平,免疫组化法测定乳腺癌组织及癌旁组织中NGF的阳性表达率。分析乳腺癌组织中miR-4500、NGF表达与患者临床病理特征及MR征象的相关性。生物信息网站预测miR-4500与NGF的靶向关系,并分析二者表达的相关性。结果与癌旁组织相比,乳腺癌组织中miR-4500表达水平降低(P<0.05),NGF mRNA水平、NGF阳性表达率升高(P<0.05)。年龄、病理类型、肿瘤分型、实质背景、表观弥散系数、强化曲线在不同miR-4500表达及NGF表达间差异无统计学意义(P>0.05),组织学分级、淋巴结转移、肿瘤直径、肿瘤形态、环形强化、瘤周水肿与miR-4500表达及NGF表达有关(P<0.05)。生物信息网站预测显示,miR-4500与NGF存在结合位点,乳腺癌组织中miR-4500表达与NGF mRNA表达呈负相关(r=-0.576,P<0.05)。结论乳腺癌组织miR-4500低表达,NGF高表达,二者与患者术前MR征象具有相关性,为MR征象提供了分子学依据。Objective To analyze the correlations of the expressions of miR-4500 and nerve growth factor(NGF)in breast cancer patients with preoperative magnetic resonance(MR)signs.Methods A total of 105 patients with breast cancer admitted to our hospital were selected,the mRNA expression levels of miR-4500 and NGF in breast cancer tissues and adjacent tissues of patients were detected by qRT-PCR,and the positive expression rates of NGF in breast cancer tissues and adjacent tissues were determined by immunohistochemistry method.The correlations of the expressions of miR-4500 and NGF in breast cancer tissues with clinicopathological features and MR signs of patients were analyzed.The targeting relationship between miR-4500 and NGF was predicted by the bioinformatics website,and the correla‐tion between the expressions of the two was analyzed.Results Compared with the adjacent tissues,the expression level of miR-4500 in breast cancer tissue decreased(P<0.05),while the level of NGF mRNA and the positive expression rate of NGF increased(P<0.05).There was no significant difference in age,pathological type,tumor classification,parenchymal background,apparent diffusion coefficient or enhancement curve among different expressions of miR-4500 and NGF(P>0.05).The histological grade,lymph node metastasis,tumor diameter,tumor morphology,ring-like enhancement,and peritu-moral brain edema were related to the expressions of miR-4500 and NGF(P<0.05).The bioinformatics website prediction showed that miR-4500 and NGF had binding sites,and the expressions of miR-4500 and NGF mRNA in breast cancer tissues were negatively correlated(r=-0.576,P<0.05).Conclusion The expression of miR-4500 in breast cancer tissue is low,and the expression of NGF is high,which are correlated with the preoperative MR signs in patients,providing a molecu‐lar basis for MR signs.

关 键 词：乳腺癌 miR-4500 神经生长因子 MR征象 

分 类 号：R445.2[医药卫生—影像医学与核医学] R737.9[医药卫生—诊断学]