伴栽天麻蜜环菌最佳原种培养基筛选  

Screening of the Best Original Strain Culture Medium of Armillaria mellea with Gastrodia elata as Companion

作　　者：李健 王鑫[1] 杜忠伟[1] 王旭[1] 王斌 胡志强[1] Li Jian;Wang Xin;Du Zhongwei;Wang Xu;Wang Bin;Hu Zhiqiang(Yanbian Academy of Agricultural Sciences,Yanji Experimental Station,CARS,Yanji,Jilin 133000,China;Wanging County Agriculture and Rural Affaris Bureau,Wangqing,Jilin 133200,China)

机构地区：[1]延边朝鲜族自治州农业科学院,国家食用菌产业技术体系延吉综合试验站,吉林延吉133000 [2]汪清县农业农村局,吉林汪清133200

出　　处：《东南园艺》2023年第5期358-362,F0003,共6页Southeast Horticulture

基　　金：财政部和农业农村部国家现代农业产业技术体系资助项目(CARS-20);延边州科技成果转化计划项目(2021NS12)

摘　　要：【目的】为筛选出伴栽天麻蜜环菌最佳原种培养基,以提高天麻产量。【方法】通过对4种伴栽天麻蜜环菌原种培养基进行比较试验。【结果】发现以枝条培养基接种后菌丝复活时间最短,平均为4.33 d,菌索生长速度为9.00mm/d,菌索长势最好,菌索长满袋时间最短,为26.33d,杂菌污染率最低为8%,培养效果优于其他3种培养基;木屑培养基、黄豆培养基和玉米粒培养基在接种后菌丝和菌索生长情况、杂菌污染率等差异均较小。【结论】综合各种因素,伴栽天麻蜜环菌最佳原种培养基为枝条培养基。[Objective]To screen out thebest original strain culturemediumof ArmillariamelleawithGastrodia elata as Companion in order to increase the yield of Gastrodia elata.[Method]The comparative test of four kinds of culture media of Armillaria mellea with Gastrodia elata were conducted.[Result]It was found that the shortest time of hypha revivification time was 4.33 days after inoculation on branch culture medium,the growth rate of rhizomorph was 9.00 mm/d,rhizomorph growth vigor was the best,and the shortest time of rhizomorph to fulfll bag was 26.33 days,and the lowest contamination rate of miscellaneous bacteria was 8%.Culture effect on branch culture medium was better than that on three other media.Sawdust culture medium,soybean culture medium and corn kernel culture medium had little difference on hypha and rhizomorph growth status and contamination rate of miscellaneous bacteria after inoculation.[Conclusion] Branch culture medium was the best culture medium of Armillaria mellea with Gastrodia elata based on comprehensive comparison of various factors.

关 键 词：天麻 蜜环菌 原种培养基 

分 类 号：S567.239[农业科学—中草药栽培]