宿主身份与叶性状对海南热带山地雨林樟科和壳斗科植物叶内生真菌群落的影响  

Effects of host identity and leaf traits on foliar endophytic fungal communities in Lauraceae and Fagaceae plants of tropical montane rainforest of Hainan Island

作　　者：罗竹慧 吴春玲 王艳丽[1] 李萃玲[2] 万玲[2] 丁琼[1] LUO Zhuhui;WU Chunling;WANG Yanli;LI Cuiling;WAN Ling;DING Qiong(School of Ecological and Environmental Sciences,Hainan University,Haikou,Hainan 570228,China;School of Horticulture,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学生态与环境学院,海口570228 [2]海南大学园艺学院,海口570228

出　　处：《热带生物学报》2024年第1期52-59,共8页Journal of Tropical Biology

基　　金：国家自然科学基金项目(31960237)。

摘　　要：为了揭示植物身份与叶性状在叶内生真菌群落构建中的作用,笔者使用Illumina Miseq测序平台检测海南尖峰岭热带山地雨林中优势植物叶内生真菌群落的物种组成,并探讨宿主身份和叶性状对叶内生真菌群落物种组成的影响。本研究共检测到来自8种樟科和7种壳斗科植物的叶内共生真菌的1539567条真菌的ITS2序列,并将它们划分为5471个真菌分类单元(Operational Taxonomic Units,OTUs),隶属于8个门、30个纲、108个目、281个科、892个属。其中,子囊菌是叶内生真菌的最大类群,占所测真菌序列数的94.5%。单株樟科植物叶内生真菌物种数805±32OTUs显著高于壳斗科植物554±41 OTUs。相比之下,Shannon-Wiener、Simpson多样性指数在两科植物的叶内生真菌间均无显著差异。偏冗余分析(partial redundancy analysis)结果表明,宿主科、属、种身份对叶内生真菌群落组成有显著的影响。去除植物性状的效应后,宿主科、属、种身份能够独立解释叶内生真菌群落物种组成的变异量分别达到2.9%、15.7%、33.7%。去除宿主植物身份的影响后,叶性状在总体上能够解释叶内生真菌群落物种组成的变异量达到21.3%(P=0.003),其中,叶钙含量、比叶面积、叶氮含量和叶钾含量对叶内生真菌群落物种组成有显著影响。上述结果表明,宿主身份和叶性状是热带山地雨林叶内生真菌群落构建的重要驱动因子。To investigate the effects of plant identity and leaf traits on foliar endophytic fungal community assembly,the species composition of foliar endophytic fungi in dominant plants in the tropical mountain rainforest in Jianfengling,Hainan Island were determined by using the Illumina Miseq sequencing method,based on which the effects of host identity and leaf traits on the species composition of foliar endophytic fungi were explored.A total of 1,539,567 fungal ITS2 sequences were obtained from the leaves of 8 species of Lauraceae plants and 7 species of Fagaceae plants,which were classified into 5,471 Operational Taxonomic Units(OTUs)belonging to 8 phyla,30 classes,108 orders,281 families,and 892 genera.Ascomycota was the largest group of the foliar endophytic fungi,accounting for 94.5%of all the fungal sequences.The number of endophytic fungal species in the leaves of individual Lauraceae plants(805±32)OTUs was significantly higher than that of Fagaceae plants(554±41)OTUs.However,there was no significant difference in Shannon-Wiener and Simpson diversity indices between the endophytic fungi in the leaves of the plants of these two families.Partial redundancy analysis showed that the host plant identities of family,genus and species significantly affected the composition of endophytic fungal communities.After removing the effect of plant traits,the host plant identities of family,genus,and species could independently explain 2.9%,15.7%,and 33.7%of the variation in endophytic fungal species composition,respectively.After removing the effect of host plant identity,leaf traits could explain 21.3%of the variation in endophytic fungal species composition(P=0.003),of which leaf calcium content,specific leaf area,leaf nitrogen content,and leaf potassium content had significant effects on the composition of endophytic fungal communities.These findings suggest that host identity and leaf traits are important factors driving the assembly of endophytic fungal communities in the tropical mountain rainforest.

关 键 词：叶内生真菌 热带山地雨林 多样性 宿主身份 叶性状 

分 类 号：Q938.1[生物学—微生物学]