周氏啮小蜂CcGSTS1基因的克隆·蛋白表达纯化及酶学特征分析  

Cloning,Expression,Purification and Enzyme Activity of CcGSTS1 from Chouioia cunea Yang

作　　者：覃东玉 任睿 孙美娣 李敏[1] 潘丽娜[1] QIN Dong-yu;REN Rui;SUN Mei-di(Tianjin Normal University/Tianjin Key Laboratory of Animal and Plant Utilization and Protec-tion,Tianjin 300387)

机构地区：[1]天津师范大学/天津市动物多样性保护与利用重点实验室,天津300387

出　　处：《安徽农业科学》2024年第2期93-96,109,共5页Journal of Anhui Agricultural Sciences

基　　金：天津市教委科研计划项目(2019KJ089)。

摘　　要：鉴定一个编码周氏啮小蜂谷胱甘肽S-转移酶序列的全长基因CcGSTS1,系统发育分析发现,该基因与丽蝇蛹集金小蜂NvGSTS6基因具有高同源性。体外表达、纯化的重组CcGSTS1可催化CDNB与GSH结合,最适反应pH为7.0。该试验为进一步研究周氏啮小蜂CcGSTS1基因功能奠定基础。In this study,we characterized a full⁃length gene encoding GST sequences CcGSTS1 from C.cunea.Phylogenetic analysis showed that CcGSTS1 shared the highest identity with NvGSTS6 of Nasonia vitripennis.The recombinant CcGSTS1 showed glutathione⁃conjugating activity to⁃ward CDNB(1⁃chloro⁃2,4⁃dinitrobenzene)and GSH(Glutathione).The optimal reaction pH value is 7.0.This study provides a foundation for further study on the function of CcGSTS1 gene in C.cunea.

关 键 词：周氏啮小蜂 谷胱甘肽S-转移酶 系统发育分析 酶活 

分 类 号：Q965.9[生物学—昆虫学]