高效液相色谱法测定橘红枇杷片中柚皮苷的含量  

Determination of Naringin in Juhong Pipa Tablets by HPLC

作　　者：张燕 Zhang Yan(Chuxiong Technician College,Chuxiong 675000,China)

机构地区：[1]楚雄技师学院,云南楚雄675000

出　　处：《云南化工》2024年第1期88-90,共3页Yunnan Chemical Technology

摘　　要：(目的)建立用高效液相色谱法测定橘红枇杷片中柚皮苷含量的方法;(方法)除去橘红枇杷片糖衣后,研细,加入甲醇,加热回流,放冷,补足损失质量,过滤,取滤液进样。色谱仪为安捷伦HP-1100型,色谱柱为Agilent ZORBAX C 18柱,紫外测定检测波长选定为283 nm,流动相为乙腈-0.1%磷酸(体积比20∶80),流速为1 mL/min。结果表明,在柚皮苷质量浓度为7.237~115.8μg/mL范围内,线性关系良好(r=0.99994,n=6),方法回收率为98.68%(n=6)。(结论)本方法准确、灵敏度高,重现性好,可作为橘红枇杷片质量控制的检测方法。Objective:To establish a HPLC method for the determination of naringin in Juhong Pipa tablets.Method:To remove the sugar coating of Juhong Pipa tablets,add methanol,heat,reflux,cool,make up weight loss,filter,and continue filtrate for sampling.The chromatograph was Agilent HP-1100 column,Agilent ZORBAX C18 column,mobile phase,acetonitrile-0.1%phosphoric acid(20∶80,V/V),flow rate was 1 mL/min,and ultraviolet detection wavelength was 283 nm.Results:The linear relationship was good in the range of 0.072-1.16μg(r=0.99994 n=6)and the recovery rate was 98.68%(n=6).Conclusion:This method has high accuracy,sensitivity and good reproducibility,and can be used for the control content determination of naringin in Juhong Pipa tablets.

关 键 词：高效液相色谱法 柚皮苷含量 橘红枇杷片 

分 类 号：R284.1[医药卫生—中药学]