HPV L1保守序列多肽抗血清降解HPV6感染能力的测试  

Test of the ability of HPV L1 conserved sequence polypeptide to antisera to degrade HPV6 infection

作　　者：邓金芳 李志英[1] 薛冰 肖长义[2] DENG Jinfang;LI Zhiying;XUE Bing;XIAO Changyi(Renhe Hospital Affiliated to Three Gorges University,Obstetrics and Gynecology,Institute of Gynecology and Oncology,Three Gorges University,Yichang 443000,China;Teaching and Research Department of Histology and Embryology,School of Basic Medicine,Three Gorges University,Yichang 443000,China)

机构地区：[1]三峡大学附属仁和医院妇产科,三峡大学妇科肿瘤研究所,宜昌443000 [2]三峡大学基础医学院组织学与胚胎学教研室,宜昌443000

出　　处：《中国免疫学杂志》2024年第1期78-81,共4页Chinese Journal of Immunology

基　　金：国家卫生健康委医药卫生科技发展研究中心项目(HDSL202004006);湖北省卫生健康委员会联合基金项目(WJ2019H535)。

摘　　要：目的:明确对不同类型人乳头瘤病毒主要衣壳蛋白(HPV L1)具有交叉反应性的HPV L1 C-末端保守序列抗体是否具有降解HPV6感染能力的作用。方法:收集尖锐湿疣标本,从收集的标本中找出HPV6型感染病例,并提取病毒。将多肽抗血清按不同比例稀释,与得到的病毒共同培养并中和,接触单层培养的人永生化角质形成细胞,PCR检验经HPV6病毒接触后人永生化角质形成细胞中HPV6 DNA含量,ELISA检验此细胞中HPV6 L1蛋白存在与否。结果:经不同稀释浓度抗血清中和的HPV6病毒感染的人永生化角质形成细胞,其DNA提取物的PCR产物经电泳,在凝胶280 bp处,HPV6特异性区间呈现不同强度的阳性条带,阳性条带的强度随抗血清浓度的升高逐渐降低。ELISA对经抗血清中和病毒接触的人永生化角质形成细胞提取蛋白进行测试,HPV L1蛋白表达量显示出与上述PCR检测结果同样的梯度变化趋势,变化差异具有统计学意义。结论:初步证明,HPV6 L1保守序列多肽抗血清可部分降解该病毒的感染,该抗血清具有针对更多型HPV进行中和研究的价值。Objective:To determine whether human papillomavirus(HPV L1)C-terminal conserved sequence antibodies with cross-reactive major capsid proteins of different types of HPV L1 have the ability to degrade HPV6 infection.Methods:Condyloma specimens were collected,HPV6 infection cases were identified from the collected samples,and virus was extracted.Polypeptide anti-sera were diluted in different proportions,and then co-cultured and neutralized with the resulting virus,then removed to contact mono-layer-cultured human immortalized keratinocytes and tested by HPV6 disease using PCR.Content of HPV6 DNA in human immortalized keratinocytes was exposed,and the presence of HPV6 L1 protein in this cells was tested by ELISA.Results:Human immortalized ke-ratinocytes infected with HPV6 virus neutralization at different dilution concentrations,the PCR products of their DNA extracts were electrophoresis and showed positive bands of HPV6 specificity zone at 280 bp of the gel,and the intensity of positive bands gradually decreased with increasing antiserum concentration.Protein extracted from human immortalized keratinocytes exposed to anti-serum neutralizing virus was tested by ELISA,and the amount of HPV L1 protein showed the same gradient trend as the above PCR test results,and the difference were statistically significant.Conclusion:It is preliminarily proved that HPV6 L1 conserved sequence polypeptide antisera can partially degrade the infection ability of the virus,and it has the value of studying more HPV neutralization types.

关 键 词：HPV 多肽抗血清 人永生化角质形成细胞 降解 体外感染 

分 类 号：R392.7[医药卫生—免疫学]