百合LoXTH23基因克隆、生物信息学分析及在鳞茎休眠解除过程中的表达动态  

Cloning,Bioinformatics Analysis and Expression Dynamics of LoXTH23 Gene in Bulb Dormancy Release Process of Lily

作　　者：张济民 王浩 贾文杰[2] 孙亮 张小平[1] 王有国[1] ZHANG Jimin;WANG Hao;JIA Wenjie;SUN Liang;ZHANG Xiaoping;WANG Youguo(College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,China;Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;School of Agriculture,Jinhua Polytechnic,Jinhua 321016,China)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南省农业科学院花卉研究所,云南昆明650205 [3]金华职业技术学院农学院,浙江金华321016

出　　处：《云南农业大学学报（自然科学版）》2023年第6期1041-1048,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(32060690)。

摘　　要：【目的】研究西伯利亚百合(Lilium oriental ‘Siberia’)休眠解除过程中LoXTH23基因的作用。【方法】以西伯利亚百合鳞茎为试验材料,从前期转录组测序结果中筛选出1个休眠解除期差异显著的XTH家族基因,利用RT-PCR技术扩增该基因cDNA全长序列,命名为LoXTH23。采用生物信息学软件对LoXTH23基因结构、理化性质等进行分析;采用荧光定量PCR技术测定LoXTH23基因在鳞茎休眠解除过程中的相对表达量。【结果】LoXTH23基因开放阅读框全长858 bp,编码285个氨基酸,包含GH16结构域,属于XTH家族基因;编码的蛋白相对分子量约为3.19 ku,理论等电点为6.19,是存在跨膜结构域和信号肽的不稳定亲水蛋白;二级结构中以随机卷曲为主,存在30个磷酸化位点;荧光定量PCR结果显示:LoXTH23基因的表达量在鳞茎休眠完全解除10 d时最高。【结论】LoXTH23可能在百合鳞茎休眠解除调控进程中起重要作用。[Purpose]To study the role of LoXTH23 gene in the process of dormancy release in Lilium oriental‘Siberian’.[Methods]The bulbs of L.oriental‘Siberian’were used as test materi-als,and a XTH family gene with significant differences in dormancy release period was screened out from the results of pre-transcriptome sequencing,which amplified the full-length sequence of the cDNA by RT-PCR,named LoXTH23.Bioinformatics software was used to analyse the structure,physicochemical properties,etc.of the LoXTH23 gene,and fluorescence quantitative PCR was used to determine the relative expression level of the LoXTH23 gene in the process of bulb dormancy release.[Results]The open reading frame of LoXTH23 gene was 858 bp in length,encoding 285 amino acids,including GH16 structural domain,and belonging to the XTH family of genes;the relative mo-lecular weight of the encoded protein was about 3.19 ku,and the theoretical isoelectric point was 6.19;it was an unstable hydrophilic protein with the presence of transmembrane structural domains and a signal peptide;random coiling was dominant in the secondary structure,and there were 30 phos-phorylation sites.The results of fluorescence quantitative PCR showed that the expression level of LoXTH23 gene was the highest at 10 days,when the bulb dormancy was completely released.[Conclusion]LoXTH23 may play an important role in the regulation of lily bulb dormancy release.

关 键 词：百合 LoXTH23 生物信息学分析 休眠解除 

分 类 号：S682.265.01[农业科学—观赏园艺]