红茎与绿茎紫花苜蓿色素及缩合单宁含量的比较分析  

Comparative Analysis of Pigment and Condensed Tannin Content between Red-stem and Green-stem Alfalfa

作　　者：宗亚倩 全伟[2] 李建平 何承刚[1] 姜华[1] ZONG Yaqian;QUAN Wei;LI Jianping;HE Chenggang;JIANG Hua(Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Rural Science and Technology Service Center,Kunming 650021,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省农村科技服务中心,云南昆明650021

出　　处：《云南农业大学学报（自然科学版）》2023年第6期1067-1072,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省农业联合基金重点项目(202101BD070001-026);云南省技术创新人才(2018HB075);国家自然科学基金项目(31660682)。

摘　　要：【目的】探究红茎紫花苜蓿的色素成分和呈色机理。【方法】以红茎和绿茎紫花苜蓿为材料,采用化学显色法和紫外分光光度计法测定茎和叶中的色素种类以及叶绿素、类胡萝卜素、花青素苷和缩合单宁的含量。【结果】红茎紫花苜蓿茎中主要含花青素苷和类黄酮,绿茎紫花苜蓿茎中不含花青素苷;红茎和绿茎紫花苜蓿叶中的叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量均显著高于茎中的含量(P<0.05),但不同颜色茎间、叶间的差异不显著(P>0.05);红茎紫花苜蓿茎中的花青素苷和缩合单宁含量及花青素苷与总叶绿素比值均显著高于绿茎紫花苜蓿茎中的对应指标(P<0.05)。【结论】花青素苷和缩合单宁含量增加是紫花苜蓿茎秆呈现红色的主要原因。[Purpose]To explore the pigment composition and color mechanism of red-stem alfalfa.[Methods]Red-stem and green-stem alfalfa were used as materials,the pigment components,and the contents of chlorophyll,carotenoid,anthocyanin and condensed tannin in stems and leaves were determined by chemical chromogenic method and spectrophotometer.[Results]The stem of red-stem alfalfa mainly contained anthocyanin and flavonoids,and the stem of green-stem alfalfa had not anthocyanin.The contents of chlorophyll a,chlorophyll b,total chlorophyll and carotenoid were signi-ficantly higher in leaves of red-stem and green-stem alfalfa than those in stems(P<0.05).However,the differences between different colors of stems and leaves were not significant(P>0.05).The con-tents of anthocyanin glycosides and condensed tannins,and ratio of anthocyanin glycosides to total chlorophyll in the stem of red-stem alfalfa were significantly higher than those in the stem of green-stem alfalfa(P<0.05).[Conclusion]The increase of anthocyanin and condensed tannin contents are the main reason for red coloration of alfalfa stems.

关 键 词：紫花苜蓿 叶绿素 类胡萝卜素 花青素苷 缩合单宁 

分 类 号：S963.223.3[农业科学—水产养殖]