旋毛虫钙网蛋白对小鼠巨噬细胞和大鼠PBMC免疫功能的影响  

Effect of Trichinella spiralis calreticulin on immune function of mouse macrophages and rat PBMC

作　　者：张朝莹 文兆海 张玥 李嫄 徐立新[1] 陆明敏 宋小凯[1] 李祥瑞[1] 严若峰[1] ZHANG Zhaoying;WEN Zhaohai;ZHANG Yue;LI Yuan;XU Lixin;LU Mingmin;SONG Xiaokai;LI Xiangrui;YAN Ruofeng(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《南京农业大学学报》2024年第1期1-8,共8页Journal of Nanjing Agricultural University

基　　金：江苏省社会发展项目(BE2022848)。

摘　　要：[目的]本文旨在研究旋毛虫钙网蛋白(TsCRT)对宿主免疫系统的影响。[方法]将原核表达纯化的重组旋毛虫钙网蛋白(rTsCRT)进行Western Blot验证;将rTsCRT与小鼠巨噬细胞共孵育,检测该蛋白对小鼠巨噬细胞增殖、NO释放和凋亡的影响;将rTsCRT与大鼠外周血单核细胞(PBMC)共孵育,检测该蛋白对大鼠PBMC转录因子和细胞因子的影响。[结果]Western blot结果显示,rTsCRT可以被感染旋毛虫的大鼠血清所识别;rTsCRT可促进小鼠巨噬细胞的增殖和NO的分泌,抑制小鼠巨噬细胞的凋亡;rTsCRT下调大鼠PBMC转录因子T-bet、Gata-3和PU.1的转录水平,上调Foxp3、Ahr、RORγt和Bcl-6的转录水平;rTsCRT上调大鼠PBMC细胞因子IFN-γ、IL-17、IL-10、TGF-β、Il-9和IL-22的转录水平,下调IL-4和IL-21的转录水平。[结论]旋毛虫钙网蛋白可刺激宿主产生Th1、Th17、Treg、Th9和Th22等多种类型的免疫反应,抑制Th2和Tfh免疫,对宿主免疫调节作用复杂。[Objectives]This paper was to study the effect of Trichinella spiralis calreticulin(TsCRT)on host immune system.[Methods]The recombinant calreticulin of T.spiralis(rTsCRT)was expressed by prokaryotic expression and purified,which was analysed by Western blot.Cells proliferation,apoptosis and NO releasing were detected after rTsCRT co-incubating with mouse macrophage.The transcription factors and cytokines were detected after rTsCRT co-incubating with rat peripheral blood mononuclear cells(PBMC).[Results]Western blot showed that rTsCRT could be recognized by the sera of rat infected with T.spiralis.Cell proliferation and NO secretion of mouse macrophage were promoted.Mouse macrophage apoptosis was inhibited by rTsCRT.The mRNA levels of T-bet,Gata-3 and PU.1 were down-regulated in rat PBMC.Foxp3,Ahr,RORγt and Bcl-6 mRNA levels were up-regulated.The mRNA levels of IFN-γ,IL-17,IL-10,TGF-β,IL-9 and IL-22 mRNA levels were up-regulated in rat PBMC.IL-4 and IL-21 were down-regulated.[Conclusions]Th1,Th17,Treg,Th9 and Th22 immune responses of host were produced.Th2 and Tfh immunity were inhibited by T.spiralis calreticulin.rTsCRT had a complex effect on host immune regulation.

关 键 词：旋毛虫 钙网蛋白 巨噬细胞 PBMC 免疫调节 

分 类 号：S855.91[农业科学—临床兽医学]