基于TMT蛋白组学分析姜黄素对热应激蛋鸡肝脏抗氧化性能的影响  

Effects of curcumin on the antioxidative properties of the liver of heat-stressed laying hens based on TMT proteomics

作　　者：杨铭宣 刘梦杰 王晋 虞德兵[1] YANG Mingxuan;LIU Mengjie;WANG Jin;YU Debing(College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《南京农业大学学报》2024年第1期96-104,共9页Journal of Nanjing Agricultural University

基　　金：江苏省农业科技自主创新资金项目(CX[22]3196)。

摘　　要：[目的]本试验旨在研究日粮添加姜黄素对由热应激引起的蛋鸡肝脏抗氧化性能损伤的影响。[方法]以南京盘滁机械化养鸡场提供的180只280日龄海兰蛋鸡为研究对象,随机分成3组:对照组(Con)饲喂基础饲粮,环境温度为(24±2)℃;热应激组(HS)是基于对照组的基础上每天维持热应激6 h以上,环境温度为(34±2)℃;姜黄素组(HS+CUR)是在热应激组的基础上,饲喂基础饲粮中添加150 mg·kg^(-1)姜黄素的试验饲粮;每组4个重复,每个重复15只鸡。采集蛋鸡肝脏组织样利用TMT(tandem mass tags)蛋白质组学技术分析蛋鸡肝脏蛋白质差异表达。[结果]实时荧光定量PCR结果显示:热应激组与对照组相比肝脏SOD1和GPX1的表达极显著降低(P<0.01),CAT表达水平显著降低(P<0.05);添加姜黄素可以极显著增加肝脏SOD1和GPX1表达水平(P<0.01),显著增加CAT表达水平(P<0.05)。TMT蛋白组学分析共检测到5 039个蛋白,其中4 235个具有定量信息,差异表达蛋白主要参与脂质和葡萄糖代谢、氧化应激、线粒体功能障碍和炎症反应等生物进程;主要参与的途径有PPAR信号通路、过氧化物酶体、脂质代谢、细胞色素P450、生物合成以及脂肪细胞因子信号通路等。选取15个显著表达的蛋白进行基因水平上的验证,结果表明基因水平和蛋白组学分析结果一致。[结论]补充姜黄素可以缓解蛋鸡由热应激引起的肝脏氧化应激以及与脂质代谢相关生物学过程。[Objectives]The purpose of this experiment was to investigate the effect of dietary curcumin supplementation on the damage of liver antioxidant properties in laying hens caused by heat stress.[Methods]One hundred and eighty 280-day-old Hylan laying hens provided by the Nanjing Panchu Mechanized Chicken Farm were taken as the research objects,and they were randomly divided into 3 groups.The control group(Con)was fed a basal diet and the ambient temperature was(24±2)℃;the heat stress group(HS)was based on the control group and maintained heat stress for more than 6 hours a day,the ambient temperature was(34±2)℃;curcumin group(HS+CUR)was based on the heat stress group and was fed basal diet supplemented with 150 mg·kg~(-1) curcumin;4 repetitions per group,15 chickens per replicate.In order to explore the protective effect of curcumin on the liver of heat-stressed laying hens,TMT(tandem mass tags)proteomics technology was used to analyze the differential expression of liver proteins in laying hens.[Results]The results of real-time fluorescence quantitative PCR showed that the expressions levels of SOD1 and GPX1 in the liver of the heat stress group were significantly lower than those of the control group(P<0.01),and the expression level of CAT significantly reduced(P<0.05);after adding curcumin,the expression levels of SOD1 and GPX1 in the liver significantly increased(P<0.01),and the expression level of CAT significantly increased(P<0.05).A total of 5 039 proteins were detected by TMT proteomic analysis,of which 4 235 had quantitative information.The results of TMT showed that the differentially expressed proteins were mainly involved in lipid and glucose metabolism,oxidative stress,mitochondrial dysfunction,and inflammation.The KEGG enrichment analysis showed that the PPAR signaling pathway,peroxidase Body,lipid metabolism,cytochrome P450,biosynthesis and adipocytokine signaling pathways were significantly enriched among groups.Fifteen differentially expressed proteins were selected for gene level verificati

关 键 词：姜黄素 鸡 热应激 肝脏 氧化应激 脂质代谢 

分 类 号：S831[农业科学—畜牧学]