维甲酸对热应激小鼠睾丸损伤的保护作用  

Protective effect of retinoic acid on testicular injury in heat stressed mice

作　　者：石一凡 李晓彤 锁云鹏 刘俊泽 李春梅[1] 李延森[1] SHI Yifan;LI Xiaotong;SUO Yunpeng;LIU Junze;LI Chunmei;LI Yansen(Research Center for Livestock Environmental Control and Smart Production,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学动物科技学院家畜环境控制与智慧生产研究中心,江苏南京210095

出　　处：《南京农业大学学报》2024年第1期183-192,共10页Journal of Nanjing Agricultural University

基　　金：中国博士后科学基金(2020M681648);江苏省博士后科研资助计划(2021K435C);江苏现代农业(生猪)产业技术体系建设项目(JATS[2022]083)。

摘　　要：[目的]本试验旨在探讨维甲酸对热应激小鼠睾丸损伤的保护作用。[方法]试验选用40只7周龄雄性ICR小鼠,随机分为4组:对照组(CON)、热应激组(HTRA_(0))、5 mg·kg^(-1)·d^(-1)维甲酸处理组(HTRA_(5))和10 mg·kg^(-1)·d^(-1)维甲酸处理组(HTRA_(10))。小鼠灌胃相应剂量维甲酸或玉米油后,42℃全身热应激2 h,处理7 d,试验结束后屠宰采样。[结果]与CON组相比,HTRA_(0)组小鼠睾丸重、最小直径以及生精小管的横截面积、直径和上皮厚度显著降低(P<0.05),附睾精子活力显著下降(P<0.05),丙二醛(MDA)含量显著升高(P<0.05),总超氧化物歧化酶(T-SOD)活性显著降低(P<0.05),抗氧化相关基因(nrf2、ho-1、nqo1、sod1、gpx1)表达量显著增加(P<0.05),bax基因表达量显著升高(P<0.05),血睾屏障相关基因(occludin、zo-1、n-cadherin和connexin 43)表达显著增加(P<0.05),精子发生相关基因(plzf、c-kit)表达量显著增加(P<0.05)。与HTRA_(0)比,HTRA_(5)组小鼠生精小管的横截面积、直径和上皮厚度显著升高(P<0.05),MDA含量显著降低(P<0.05),T-SOD活性显著升高(P<0.05),plzf和c-kit mRNA表达量显著降低(P<0.05);HTRA_(10)组小鼠生精小管的横截面积、直径和上皮厚度显著升高(P<0.05),精子的活力显著提高(P<0.05),MDA含量显著降低(P<0.05),T-SOD活性显著提高(P<0.05),nqo1、bax、bcl-2、zo-1、n-cadherin、plzf和c-kit基因表达量显著降低(P<0.05)。[结论]维甲酸能够改善热应激小鼠的睾丸重、睾丸组织形态和附睾精子活力,使之更接近对照组水平;这种保护作用可能是通过增加小鼠睾丸抗氧化能力、减少生精细胞凋亡、增强血睾屏障、改善生精微环境实现的。[Objectives]The aim of this experiment was to investigate the protective effect of retinoic acid on testicular injury in heat stressed mice.[Methods]Forty 7-week-old male ICR mice were randomly divided into four groups:control group(CON),heat stressed group(HTRA_0),5 mg·kg~(-1)·d~(-1)retinoic acid-treated group(HTRA_5)and 10 mg·kg~(-1)·d~(-1)retinoic acid-treated group(HTRA_(10)).Mice were gavaged with appropriate doses of retinoic acid or corn oil and then subjected to systemic heat stress at 42 ℃ for 2 h.The mice were slaughtered and sampled at the end of the test.[Results]Compared with the CON group,the testicular weight,minimum diameter,cross-sectional area,diameter,and epithelial height of spermatogenic tubules in the HTRA_(0) group were significantly reduced(P<0.05),the epididymal sperm motility significantly decreased(P<0.05),the malondialdehyde(MDA)content and the expression levels of antioxidant related genes(nrf2,ho-1,nqo1,sod1,gpx1)in testis tissue significantly increased(P<0.05),while total superoxide dismutase(T-SOD)activity significantly decreased(P<0.05),the mRNA expression level of bax significantly increased(P<0.05),the expression levels of blood-testis barrier related genes(occludin,zo-1,n-cadherin and connexin 43)significantly increased(P<0.05),and the expression levels of spermatogenesis related genes(plzf,c-kit)expression significantly increased(P<0.05).Compared with HTRA_0,the cross-sectional area,diameter and epithelial height in the HTRA_(5) group significantly increased(P<0.05),the MDA content significantly decreased(P<0.05)while T-SOD activity significantly increased(P<0.05),and the mRNA expression levels of plzf and c-kit significantly decreased(P<0.05).Compared with HTRA_0,the cross-sectional area,diameter and epithelial height of spermatogenic tubules in the HTRA_(10) group significantly increased(P<0.05),sperm motility significantly increased(P<0.05),MDA content significantly decreased(P<0.05),T-SOD activity significantly increased(P<0.05),and the mRNA expression levels of nqo1

关 键 词：热应激 维甲酸 小鼠 睾丸 精子活力 氧化应激 血睾屏障 

分 类 号：S857.29[农业科学—临床兽医学]