一株中华稻蝗内生真菌Fusarium lateritium ZMT01的代谢产物  

The metabolites of an endophytic fungus Fusarium lateritium ZMT01 from Oxya chinensis Thunberg

作　　者：房豪东 黄子辉 李苏柠 黎杨倩 佘志刚[2] 熊亚红 李春远 FANG Haodong;HUANG Zihui;LI Suning;LI Yangqian;SHE Zhigang;XIONG Yahong;LI Chunyuan(College of Materials and Energy,South China Agricultural University/Key Laboratory for Biobased Materials and Energy of Ministry of Education,Guangdong Guangzhou 510642,China;School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]华南农业大学材料与能源学院/生物基材料与能源教育部重点实验室,广东广州510642 [2]中山大学化学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）（中英文）》2024年第1期71-77,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省基础与应用基础研究基金(2019B1515210023,2020A1515010325);广东省自然科学基金(2018A030313582)。

摘　　要：研究一株中华稻蝗内生真菌Fusarium lateritium ZMT01的代谢产物,采用大米培养基发酵真菌,色谱技术分离纯化单体,波谱分析鉴定结构,共从发酵物中分离鉴定了10个化合物:fusopoltide A(1)、fusopoltide B(2)、fusopoltide D(3)、solaniol(4)、javanicin(5)、(1S,4S,10S)-3,4-dihydro-6,9-dihydroxy-8-methoxy-10(-2-oxopropyl)-1,4-methano-2-benzoxepin-5(1H)-one(6)、2,2′‐methylenebis(4‐methyl‐6‐tert‐butylphenol)(7)、2-hydroxymethyl-5-isopropoxy-4-methoxynaphthalen(8)、β-sitosterol(9)和walterolactone A(10)。其中化合物8、10为首次从Fusarium属中得到,除化合物9外,其他化合物为首次从Fusarium lateritium中分离到。采用二倍稀释法测试抗菌活性,结果显示化合物4和5对O6血清型大肠杆菌Escherichia coli有强抑制活性,MIC为6.25μg/mL,化合物4对尖孢镰孢菌Fusarium oxysporum显示中等抑菌活性,MIC为200μg/mL。The metabolites of an endophytic fungus Fusarium lateritium ZMT01 from Oxya chinensis Thunbergwere investigated.The fungus was cultured in rice medium,the monomeric compounds were isolated and purified by the chromatographic technique,and the structures of compounds were identified by analysis of spectroscopy.Ten compounds were isolated and identified as fusopoltide A(1),fusopoltide B(2),fusopoltide D(3),solaniol(4),javanicin(5),(1S,4S,10S)-3,4-dihydro-6,9-dihydroxy-8-methoxy-10-(2-oxopropyl)-1,4-methano-2-benzoxepin-5(1H)-one(6),2,2′‐methylenebis(4‐methyl‐6‐tert‐butylphenol)(7),2-hydroxymethyl-5-isopropoxy-4-methoxynaphthalen(8),β-sitosterol(9)and walterolactone A(10).Among them,compounds 8 and 10 were firstly obtained from the genus Fusarium,and all of them except compound 9 were isolated from Fusarium lateritium for the first time.The antibacterial activities of these compounds were tested in vitro using the two-fold dilution method.Compounds 4 and 5 showed a high inhibitory activity against Escherichia coli with serotype O6 with MIC of 6.25μg/mL,and compound 4 showed a moderate inhibitory activity against Fusarium oxysporum with MIC of 200μg/mL.

关 键 词：内生真菌 砖红镰刀菌 代谢产物 抗菌活性 

分 类 号：O629[理学—有机化学]