枸杞多糖对心肌梗死大鼠心肌肌钙蛋白I表达及心脏微血管的影响  

Effects of Lycium Barbarum Polysaccharide on Cardiac Troponin I Expression and Cardiac Microvessel in Rats with Myocardial Infarction

作　　者：李会贤[1] 张爱爱[1] 张亚维 陈伟广 马骁 LI Hui-Xian;ZHANG Ai-Ai;ZHANG Ya-Wei;CHEN Wei-Guang;MA Xiao(Dept.of Cardiovascular Medicine,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000 Hebei,China;Zhangjiakou Municipal Health Commission,Zhangjiakou 075000 Hebei,China;Inpatient Department,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000 Hebei,China)

机构地区：[1]河北北方学院附属第一医院心血管内科,河北张家口075000 [2]张家口市卫生健康委员会,河北张家口075000 [3]河北北方学院附属第一医院住院处,河北张家口075000

出　　处：《广州中医药大学学报》2024年第2期423-428,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：河北省医学科学研究基金资助项目(编号:20231406)。

摘　　要：【目的】探讨枸杞多糖对心肌梗死大鼠的治疗作用及机制。【方法】实验将SD大鼠随机分为正常组、模型组、氯沙坦组、枸杞多糖组。除正常组,其他3组大鼠采用左冠状动脉主干结扎法建立心肌梗死模型。给予相应干预后,采用心脏彩超检查大鼠心功能[左室收缩末期内径(LVSD)、左室舒张末期内径(LVDD)、室间隔(IVS)、左心室劳损(LVS)、左室射血分数(LVEF)],苏木素-伊红(HE)染色法观察心肌组织病理形态,酶联免疫吸附法(ELISA)检测血清心肌肌钙蛋白I(cTnI)、一氧化氮(NO)含量,心脏凝胶墨汁染色法测定微血管密度,蛋白免疫印迹(Western Blot)法检测心肌组织cTnI、内皮型一氧化氮合酶(eNOS)、血小板内皮细胞黏附因子1(PECAM-1)蛋白表达。【结果】与正常组比较,模型组LVSD、LVDD、IVS、LVS均显著升高(P<0.05),LVEF显著降低(P<0.05),HE染色可见心肌组织结构破坏,心肌细胞大量坏死,心肌纤维断裂,大量炎症细胞浸润,血清NO含量显著降低(P<0.05),cTnI含量显著升高(P<0.05),心脏微血管密度显著降低(P<0.05),cTnI蛋白表达明显升高(P<0.05),eNOS、PECAM-1蛋白表达显著降低(P<0.05);与模型组比较,氯沙坦组、枸杞多糖组大鼠以上各指标均得到明显改善(P<0.05),且枸杞多糖组的改善效果均优于氯沙坦组(P<0.05)。【结论】枸杞多糖可通过抑制心肌梗死大鼠cTnI表达,改善心脏微血管损伤,发挥对心脏的保护作用。Objective To investigate the therapeutic effect and mechanism of lycium barbarum polysaccharide(LBP)on rats with myocardial infarction.Methods Experimentally,SD rats were randomly divided into normal group,model group,Losartan group and LBP group.In addition to the normal group,myocardial infarction model was established by ligation of the left coronary artery trunk in the other three groups of rats.After appropriate interventions were given,cardiac function[left ventricular systolic dimension(LVSD),left ventricular diastolic dysfunction(LVDD),interventricular septum(IVS),left ventricular strain(LVS),and left ventricular ejection fraction(LVEF)]was examined by cardiac ultrasonography,myocardial tissue pathological morphology was observed by hematoxylin-eosin(HE)staining,serum cardiac troponin I(cTnI)and nitric oxide(NO)levels were measured by enzyme-linked immunosorbent assay(ELISA),microvessel density was determined by cardiac gel ink staining,and myocardial tissue protein expressions of cTnI,endothelial-type nitric oxide synthase(eNOS),and platelet endothelial cell adhesion factor 1(PECAM-1)was measured by Western Blot.Results Compared with the normal group,LVSD,LVDD,IVS and LVS were significantly increased in the model group(P<0.05),LVEF was significantly decreased(P<0.05),HE staining showed the destruction of myocardial textile structure,a large number of necrotic cardiomyocytes,myocardial fibre rupture,and a large number of inflammatory cell infiltration,the serum NO content was significantly decreased(P<0.05),the cTnI level was significantly increased(P<0.05),cardiac microvessel density was significantly reduced(P<0.05),cTnI protein expression was significantly increased(P<0.05),and protein expressions of eNOS and PECAM-1 were significantly decreased(P<0.05);compared with the model group,the above indexes of the rats in the Losartan group and the LBP group were significantly improved(P<0.05),and the improvement effect of the LBP group was superior to that of the Losartan group(P<0.05).Conclusions LBP can in

关 键 词：枸杞多糖 心肌梗死 心肌肌钙蛋白I(cTnI) 心脏微血管 大鼠 

分 类 号：R285.5[医药卫生—中药学]