慢性萎缩性胃炎肝胃气滞证和脾胃虚弱证患者血清代谢组学特征研究  

Serum Metabolomics Characteristics of Chronic Atrophic Gastritis Patients with Liver-Stomach Qi Stagnation Syndrome and Spleen-Stomach Weakness Syndrome

作　　者：陈玉意 李娟娟[1,2] 王洪亮 郭绍举 黄彬[1,2] CHEN Yu-Yi;LI Juan-Juan;WANG Hong-Liang;GUO Shao-Ju;HUANG Bin(The Fourth Clinical Medical School of Guangzhou University of Chinese Medicine,Shenzhen 518000 Guangdong,China;Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518000 Guangdong,China)

机构地区：[1]广州中医药大学第四临床医学院,广东深圳518000 [2]深圳市中医院,广东深圳518000

出　　处：《广州中医药大学学报》2024年第1期7-16,共10页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省基础与应用基础研究基金项目(编号:2019A1515110742);广东省中医药局面上科研项目(编号:20191272,20191273);黄彬广东省名中医工作室项目(粤中医办函[2020]1号)。

摘　　要：【目的】基于非靶向代谢组学技术,分析慢性萎缩性胃炎(chronic atrophic gastritis,CAG)肝胃气滞证和脾胃虚弱证患者的代谢组学特征,发现与CAG患者中医证型相关的血清代谢差异物,为中医辨证客观化提供参考依据。【方法】纳入60例CAG患者,其中肝胃气滞证及脾胃虚弱证各30例。收集2组患者空腹肘静脉血5 mL,采用超高效液相色谱-质谱技术检测血清代谢物含量,采用主成分分析(PCA)、正交偏最小二乘法判别分析(OPLS-DA)和聚类分析等多元统计方法筛选CAG肝胃气滞证和脾胃虚弱证患者之间的差异代谢物,最后利用KEGG数据库对筛选的差异代谢物进行代谢物通路分析。【结果】通过比较CAG肝胃气滞证和脾胃虚弱证患者血清代谢物,发现CAG肝胃气滞证和脾胃虚弱证之间N-乙酰基甘氨酸、组胺、O-磷酸丝氨酸、硒甲基硒代半胱氨酸、甲基间酪氨酸等氨基酸衍生物和酪氨酸-亮氨酸-苯丙氨酸、组氨酸-丙氨酸-谷酰胺-赖氨酸、L-天冬酰胺基-L-脯氨酰-L-丝氨酸、L-异亮氨酸-L-异亮氨酸等小肽类物质代谢具有显著性差异。【结论】CAG肝胃气滞证和脾胃虚弱证患者之间存在氨基酸代谢差异,N-乙酰基甘氨酸、甲基间酪氨酸、O-磷酸丝氨酸等代谢物可能是区别CAG肝胃气滞证和脾胃虚弱证的潜在生物标志物。Objective To analyze the metabolomics characteristics of chronic atrophic gastritis(CAG)patients with liver-stomach qi stagnation and spleen-stomach weakness syndromes based on non-targeted metabolomics technology,and to identify the serum differentiated metabolites related to traditional Chinese medicine(TCM)syndrome of CAG patients,so as to provide a reference for the objectification of syndrome differentiation.Methods Sixty patients with CAG were included,including 30 cases of liver-stomach qi stagnation syndrome and 30 cases of spleen-stomach weakness syndrome.Fasting blood of 5 mL was collected from the cubital vein of patients in the two groups,and the serum levels of metabolites were detected by ultra-high-performance liquid chromatographymass spectrometry(UPLC-MS)methods.The principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA),and cluster analysis were used to screen the differentiated metabolites of CAG patients with liver-stomach qi stagnation syndrome and spleen-stomach weakness syndrome.Finally,metabolite pathway analysis was performed for the obtained differentiated metabolites using the KEGG database. Results The results for the screening of differentiated metabolites showed that significant differences of amino acidderivatives and small peptide metabolites were presented between CAG patients with liver-stomach qi stagnationsyndrome and CAG patients with spleen-stomach weakness syndrome. The amino acid derivatives consisted ofN- acetylglycine, histamine, O- phosphoserine, selenomethylselenocysteine, and methyl- tyrosine. And thesmall peptide metabolites consisted of tyrosine-leucine-phenylalanine, histidine-alanine-glutamate-lysine,L- asparagine- L- proline- L- serine, and L- isoleucine- L- isoleucine. Conclusion Differences in amino acidmetabolism exist between CAG patients with liver-stomach qi stagnation syndrome and those with spleen-stomachweakness syndrome,and metabolites such as N-acetylglycine,intermethyltyrosine,and O-phosphoserine maybe the pote

关 键 词：慢性萎缩性胃炎 中医证型 肝胃气滞证 脾胃虚弱证 代谢组学 生物标志物 

分 类 号：R259.733[医药卫生—中西医结合]