多物质联用对磷石膏中磷的固定效果与机理分析  

Analysis of fixation effect and mechanism of phosphorus in phosphogypsum bymultiple substances combination

作　　者：张晨燕 付甫刚 谭文超 杨艺琳[1] 柳听义[1] ZHANG Chenyan;FU Fugang;TAN Wenchao;YANG Yilin;LIU Tingyi(Tianjin Key Laboratory of Water Resources and Water Environment,Tianjin Normal University;School of Geography and Environmental Science,Tianjin Normal University;Power China Guiyang Survey and Design Institute Co.,Ltd.)

机构地区：[1]天津师范大学,天津市水资源与水环境重点实验室 [2]天津师范大学地理与环境科学学院 [3]中国电建集团贵阳勘测设计研究院有限公司

出　　处：《环境工程技术学报》2024年第1期194-203,共10页Journal of Environmental Engineering Technology

基　　金：天津市自然科学基金项目(18JCYBJC96600)。

摘　　要：采用微生物-植物联用的磷石膏无害化处理方法,在磷石膏渣场土壤中富集筛选出2种聚磷菌,通过鉴定得知分别是假单胞菌(Pseudomonas sp.)和恶臭假单胞菌(Pseudomonas putida)。将生石灰改性的磷石膏与少量土壤按质量比混合后,与已经配置的有机肥-微生物液体菌剂均匀混合,按不同混合比例放入渗滤土柱装置中并在表层种植高羊茅,底部收集渗滤液,通过钼酸铵分光光度法测量液体中总磷浓度变化,磷石膏:土:生石灰:有机肥:微生物:草籽质量比为7:3:0.2:0.2:0.03:0.03的组磷浸出浓度在1 mg/L以下,质量比为8:2:0.2:0.2:0.03:0.03的组浸出液总磷浓度不超过4 mg/L,对比新鲜磷石膏浸出液总磷浓度(633.9 mg/L),多物质联合处理磷石膏中磷的固定率达到99%。筛选的菌株在混合土壤中存活量最高可达6.4×10^(6)cfu/mL且活菌数量随时间保持稳定或缓慢增长趋势。为解决磷石膏堆积区磷浸出浓度超标的问题以及磷石膏无害化处理提供了有效支撑。Two phospho-accumulating bacteria were enriched and screened in the soil of the slag field by the harmless treatment method of phosphogypsum by microbial-plant combination,and the bacteria were identified as Pseudomonas sp.and Pseudomonas putida,respectively.The phosphogypsum modified by quicklime was mixed with a small amount of soil according to the mass ratio,and was evenly mixed with the already allocated organic fertilizer and microbial liquid bacteria agent.The mixture was put into the infiltration soil column device according to different mixing ratios,tall fescue was planted on the surface,and the leachate was collected at the bottom.The change of total phosphorus concentration in the liquid was measured by ammonium molybdate spectrophotometry.With phosphogypsum:soil:organic fertilizer:microorganism:grass seeds mass ratio of 7∶3∶0.2∶0.2∶0.03∶0.03,the phosphorus leaching concentration in the liquid was below 1 mg/L.The concentration of total phosphorus in the leaching solution of group 8∶2∶0.2∶0.2∶0.03∶0.03 was no more than 4 mg/L,and the concentration of total phosphorus in the leaching solution of fresh phosphogypsum was 633.9 mg/L.The fixation rate of phosphorus in the combined multi-substance treatment of phosphogypsum was more than 99%.The survival rate of the selected strains in the mixed soil was up to 6.4×10^(6) cfu/mL,and the number of viable strains maintained stable or slow growth trend over time.This experiment successfully solved the problem of excessive phosphorus leaching concentration in the phosphogypsum accumulation area,and provided effective support for harmless treatment of phosphogypsum.

关 键 词：磷石膏 假单胞菌 高羊茅 磷固定 磷浸出浓度 

分 类 号：X705[环境科学与工程—环境工程]