串内频率对高频纳秒脉冲串诱导A375黑色素瘤细胞电穿孔的影响  

Effects of Intraburst Frequency on Electroporation of A375 Melanoma Cells Induced by High-frequency Nanosecond Pulse Bursts

作　　者：徐进[1,2] 米彦 吴晓[1] 李津津 唐均英 XU Jin;MI Yan;WU Xiao;LI Jinjin;TANG Junying(State Key Laboratory of Power Transmission Equipment&System Security and New Technology,Chongqing University,Chongqing 400030,China;Huizhou Power Supply Bureau,Guangdong Power Grid Company Limited,Huizhou 516001,China;First Affiliated Hospital,Chongqing Medical Science University,Chongqing 400016,China)

机构地区：[1]重庆大学输配电及系统安全与新技术国家重点实验室,重庆400030 [2]广东电网有限责任公司惠州供电局,惠州516001 [3]重庆医科大学附属第一医院,重庆400016

出　　处：《高电压技术》2023年第12期5292-5301,共10页High Voltage Engineering

基　　金：国家自然科学基金(52077022)。

摘　　要：高频纳秒脉冲串能有效杀伤肿瘤细胞,但是其杀伤机制,特别是串内频率对诱导电穿孔效应的影响机制仍不清楚。以A375黑色素瘤细胞为研究对象,开展了细胞活性检测、短时/长时荧光染料检测以及细胞微观形态学检测等离体细胞实验,研究高频纳秒脉冲串的串内频率对细胞膜电穿孔的影响机制。细胞活性实验结果表明,高频纳秒脉冲串能有效杀伤肿瘤细胞,串内频率存在使细胞活性显著降低的阈值100k Hz;荧光染料实验结果反映细胞活性降低是由不可逆电穿孔引起,并且存在串内频率对电穿孔的累积效应;扫描电镜实验结果直接验证了高频纳秒脉冲串诱导肿瘤细胞发生了不可逆电穿孔。该实验结果为高频纳秒脉冲串杀伤肿瘤细胞的机制提供了实验依据。High-frequency nanosecond pulse bursts can effectively kill tumor cells,whereas,the killing mechanism,es-pecially the effect of the intraburst frequency on the induced electroporation,is still unclear.Taking A375 melanoma cells as the research object,we carried out in vitro cell experiments such as cell viability detection,short-term/long-term fluo-rescent dye detection,and cell micro-morphological detection to study the effect of the intraburst frequency of high-frequency nanosecond pulse bursts on cell membrane electroporation.The results of cell viability experiments show that high-frequency nanosecond pulse bursts can effectively kill cells,and the intraburst frequency has a threshold of 100 kHz that significantly reduces cell viability.The results of fluorescent dye experiments reveal that the decrease in cell via-bility is caused by irreversible electroporation,and there is a cumulative effect of intraburst frequency on electroporation.The results of scanning electron microscopy directly verify that the high-frequency nanosecond pulse bursts induce irre-versible electroporation of tumor cells.The experimental results in this paper provide an experimental basis for the mechanism of high-frequency nanosecond pulse bursts killing tumor cells.

关 键 词：高频纳秒脉冲串 串内频率 A375黑色素瘤细胞 不可逆电穿孔 累积效应 

分 类 号：R739.5[医药卫生—肿瘤]