Disease burden,antimicrobial resistance and molecular characterization of invasive group B Streptococcus among non-pregnant adults in Malaysia:A protocol study  

作　　者：Abdulrahman Muthanna Nurul Hana Zainal Baharin Mohd Nasir Mohd Desa Nurul Diana Dzaraly Mohammad Noor Amal Azmai Syafinaz Amin-Nordin 

机构地区：[1]Department of Biomedical Sciences,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,43400 Serdang,Selangor,Malaysia [2]Department of Biology,Faculty of Science,Universiti Putra Malaysia,43400 Serdang,Selangor,Malaysia [3]Aquatic Animal Health and Therapeutics Laboratory,Institute of Bioscience,Universiti Putra Malaysia,43400 Serdang,Selangor,Malaysia [4]Department of Medical Microbiology,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,43400 Serdang,Selangor,Malaysia [5]Department of Medical Microbiology,Sultan Abdul Aziz Shah Hospital,Universiti Putra Malaysia,Persiaran MARDI-UPM,43400,Selangor,Malaysia [6]Laboratory of Vaccine and Biomolecules,Institute of Bioscience,Universiti Putra Malaysia,43400 Serdang,Selangor,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2023年第11期498-505,共8页亚太热带医药杂志（英文版）

基　　金：funded by the Ministry of Higher Education under Fundamental Research Grant Scheme(FRGS/1/2023/WAB04/UPM/01/4).

摘　　要：and pili genes are also investigated.Methods:This multicentre,prospective,observational study is conducted in seven major tertiary hospitals in Malaysia among non-pregnant adults.Simultaneously,a retrospective study is conducted in the selected hospitals with similar approaches.GBS isolates are subjected to phenotyping,serotyping by multiplex PCR,antimicrobial susceptibility testing and PCR-detection of GBS virulence and pilus genes.Seven housekeeping genes are amplified and sequenced for multi-locus sequence typing.Discussion:Findings from the study may contribute to the management of clinical practice to diagnose and prevent GBS related diseases in a timely manner.Prudent use of antibiotics is encouraged by monitoring antimicrobial resistance.

关 键 词：Group B Streptococcus Incidence SEROTYPE Antimicrobial resistance Virulence gene Multi-locus sequence typing 

分 类 号：R515[医药卫生—内科学] R181.3[医药卫生—临床医学]