琼脂扩散法用于放线菌源生防菌剂筛选的活性影响因素  

The influencing factors for bio-fungicide screening from actinomycetes source with agar diffusion method

作　　者：张志刚[1] 王开梅[1] 吴兆圆 万中义[1] 方伟[1] ZHANG Zhi-gang;WANG Kai-mei;WU Zhao-yuan;WAN Zhong-yi;FANG Wei(Hubei Biopesticide Engineering Research Center/The Branch Center of Biopesticide,Hubei Agricultural Scientific and Technological Innovation Center,Wuhan 430064,China)

机构地区：[1]湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药分中心,武汉430064

出　　处：《湖北农业科学》2023年第12期78-84,199,共8页Hubei Agricultural Sciences

基　　金：国家重点研发计划重点专项(2017YFD0201301-4);湖北省重点研发计划项目(2021BBA081);湖北省农业科技创新中心创新团队项目(2019-620-000-001-27)。

摘　　要：以灰霉病菌(Botrytis cinerea)、胶孢炭疽菌(Colletotrichum gloeobosporioides)、番茄枯萎病菌(Fusarium oxysporum)、西瓜猝倒病菌(Pythium aphanidematum)为模式靶标,探讨了放线菌源生防杀菌剂筛选的活性特征、影响活性稳定性和敏感度的因素。采用琼脂扩散法,测试了模式靶标、化合物标准样品和放线菌标准菌株的发酵液样品对杀菌活性测试的影响。结果表明,靶标的处理过程、靶标菌的含量、样品的类型以及样品的加入量与活性反应有直接关联。不同模式靶标菌对破碎处理的耐受程度不同,必须针对不同模式靶标进行个性化处理,以保证模式靶标菌在测试中的稳定生长状态。在琼脂扩散法中,不仅可以检测样品的杀菌活性强度,而且活性成分与靶标菌的不同作用方式也可以呈现。保留活菌的放线菌发酵液样品在加入量较宽的范围内都显示活性,而经过滤除菌处理的放线菌发酵液样品和标准化合物都只在样品加入量比较大时才显示出活性。Using Botrytis cinerea,Colletotrichum gloeobosporioides,Fusarium oxysporum and Pythium aphanidematum as the model targets,the activity characteristics and the factors affecting the stability and sensitivity of the bactericide were tested.The influence of the model target,compound standard sample and fermentation liquid sample of actinomycetes source standard strain on bactericidal activity was tested by agar diffusion method.The results showed that the treatment process of the target,the content of the target bacte⁃ria,the type of sample,and the amount of sample added were directly related to the activity response.Different model targets had dif⁃ferent degrees of tolerance to crushing treatment,so it was necessary to conduct personalized treatment for different model targets to en⁃sure the stable growth state of model target bacteria in the test.In the agar diffusion method,not only the bactericidal activity of the sample could be detected,but also the different modes of action of the active ingredient and the target bacteria could be shown.The ac⁃tinomycete fermentation broth with retained live bacteria showed activity over a wide range of addition amounts,whereas both the acti⁃nomycete fermentation broth treated with filtered bacteria and the standard compounds showed activity only when the sample addition amounts were relatively large.

关 键 词：模式靶标 生防杀菌剂 筛选 琼脂扩散法 抑菌圈 放线菌源 

分 类 号：S482.292[农业科学—农药学]