阳春砂与草果叶片解剖结构分析  

Analysis of leaf anatomical structure of Amomum villosum and Amomum tsao-ko

作　　者：杨加珍[1] 徐鹤萌 陈佳[1] 何伟 曾亚文[1] YANG Jia-zhen;XU He-meng;CHEN Jia;HE Wei;ZENG Ya-wen(Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences/Key Laboratory of Agricultural Biotechnology of Yunnan Province/Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Kunming 650205,China;Pu’er University,Pu’er 665000,Yunnan,China;Honghe Linyuan Agricultural Technology Development Co.,Ltd.,Hekou 661300,Yunnan,China)

机构地区：[1]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业农村部西南作物基因资源与种质创制重点实验室,昆明650205 [2]普洱学院,云南普洱665000 [3]红河麟源农业科技发展有限公司,云南河口661300

出　　处：《湖北农业科学》2023年第12期102-107,共6页Hubei Agricultural Sciences

基　　金：云南省乡村振兴示范项目(202304BP090002)。

摘　　要：为揭示豆蔻属(Amomum L.)2种植物阳春砂(Amomum villosum Lour.)与草果(Amomum tsao-ko Crevost et Lemaire)的叶解剖结构特性差异,采用指甲油印痕法和超薄切片法对其叶片进行研究。结果表明,2种植物的叶片在气孔器的分布、气孔器的结构、叶片横切面结构、叶肉细胞超微观结构上有很大的相似性,但在气孔器的数量、气孔器的大小、叶绿体的结构、叶肉细胞贮藏的营养物质等方面存在差异:草果叶片的上、下表皮气孔分别比阳春砂叶片的上、下表皮气孔大;阳春砂叶片的上、下表皮气孔数目分别比草果叶片的上、下表皮气孔数目多;阳春砂叶片栅栏组织和海绵组织分化不太明显,草果叶片的栅栏组织和海绵组织的分化较明显,且栅栏组织排列更加整齐、规则;阳春砂的叶绿体中含有淀粉粒和嗜锇颗粒,细胞质中含有内生菌,草果叶片的叶绿体中含有脂滴。In order to reveal the difference of leaf anatomical structure characteristics between Amomum villosum and Amomum tsaoko,the nail polish and the ultrathin slice methods were used to study the leaves.The results showed that the leaves of the two species were very similar in the distribution of stomatal organs,the structure of stomatal organs,the structure of leaf cross section,and the ul⁃tramicrostructure of mesophyll cells,but there were differences in the number of stomatal organs,the size of stomatal organs,the structure of chloroplasts,and the nutrients stored in mesophyll cells:The upper and lower epidermal stomata of Amomum tsao-ko leaves were larger than those of Amomum villosum leaves;the number of stomata in the upper and lower epidermis of the leaves of Amo⁃mum villosum was higher than that in the leaves of Amomum tsao-ko;the differentiation of palisade tissue and spongy tissue was not obvious in the leaves of Amomum villosum,but the differentiation of palisade tissue and spongy tissue was more obvious in the leaves of Amomum tsao-ko,and the palisade tissue was more orderly and regular;the chloroplasts of Amomum villosum contained starch grains and osmophilic granules,and the cytoplasm contained endophytes.The chloroplasts of Amomum tsao-ko leaves contained lipid droplets.

关 键 词：阳春砂(Amomum villosum Lour.) 草果(Amomum tsao-ko Crevost et Lemaire) 叶片解剖结构 

分 类 号：S311[农业科学—作物栽培与耕作技术]