棕黑锦蛇鳞片特征、脂肪酸和肌肉单糖分析  

Analysis of scales,fatty acids and muscle monosaccharides of Elaphe schrenckii

作　　者：黄笑然 杨文建 万祥旭 周思宇 周宝丽 孟鑫 金志民[1] HUANG Xiao-ran;YANG Wen-jian;WAN Xiang-xu;ZHOU Si-yu;ZHOU Bao-li;MENG Xin;JIN Zhi-min(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《湖北农业科学》2023年第12期141-144,150,共5页Hubei Agricultural Sciences

基　　金：黑龙江省自然基金项目(LH2020C071;ss2021c006);牡丹江师范学院项目(2021H03)。

摘　　要：为鉴定棕黑锦蛇(Elaphe schrenckii)鳞片特征,测定蛇油中脂肪酸和肌肉单糖的种类和相对含量,采用超声波辅助提取法得到蛇油和肌肉多糖,通过气相色谱仪分析甲酯化的蛇油中脂肪酸的种类和相对含量;同时,多糖经水解和衍生后通过气相色谱法分析肌肉中单糖的种类和相对含量。结果表明,不饱和脂肪酸与饱和脂肪酸的比值为2.02,不饱和脂肪酸占比较高,脂肪酸相对含量由大到小依次是油酸(34.200%)、棕榈酸(22.600%)、亚油酸(22.200%)、硬脂酸(5.280%)。肌肉多糖中单糖主要有葡萄糖和葡萄糖醛酸2种,其中葡萄糖占比69.13%,葡萄糖醛酸占比14.32%。In order to identify the scales of Elaphe schrenckii and determine the type and relative content of fatty acids in snake oil and monosaccharides in muscle,the snake oil and muscle polysaccharide were obtained by ultrasonic-assisted extraction,and the methyl esterified snake oil was analyzed by gas chromatography to obtain the types and relative contents of fatty acids;the polysaccharide was measured by gas chromatography after hydrolysis and derivatization to obtain the data of monosaccharide in muscle.The results showed that the ratio of unsaturated fatty acids to saturated fatty acids was 2.02,with a relatively high proportion of unsaturated fatty acids,and the relative content of fatty acids was oleic acid(34.200%),palmitic acid(22.600%),linoleic acid(22.200%)and stearic acid(5.280%)in descending order.The monosaccharides in muscle polysaccharides were 2 types of glucose and glucuronic acid,with glu⁃cose accounting for 69.13%and glucuronic acid for 14.32%.

关 键 词：棕黑锦蛇(Elaphe schrenckii) 鳞片 脂肪酸 单糖 

分 类 号：Q956[生物学—动物学]