酸性染料比色法测定对叶百部中总生物碱含量  

Determination of total alkaloid in Stemona tuberosa Lour.by acid dye colorimetric method

作　　者：吴港圆 覃海琴 胡东南[3] 张淼[2] 谢凤凤 王孝勋 WU Gang-yuan;QIN Hai-qin;HU Dong-nan;ZHANG Miao;XIE Feng-feng;WANG Xiao-xun(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Key Laboratory of Zhuang Yao Medicine in Guangxi,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China)

机构地区：[1]广西中医药大学药学院,南宁530200 [2]广西中医药大学广西壮瑶药重点实验室,南宁530200 [3]广西壮族自治区药用植物园,南宁530023

出　　处：《湖北农业科学》2023年第12期154-157,共4页Hubei Agricultural Sciences

基　　金：广西壮瑶药重点实验室基金资助项目(桂科基字[2014]32号);广西2011协同创新中心基金资助项目(桂教科研[2013]20号);广西南宁市科学研究与技术开发计划基金资助项目(20193120)。

摘　　要：采用酸性染料比色法测定不同发育时期的对叶百部(Stemona tuberosa Lour.)中总生物碱含量,采用酸性染料比色法,以对叶百部碱为对照品,溴麝香草酚蓝为酸性染料,甲醇、三氯甲烷提取,4%盐酸溶解,在413 nm波长下测定。结果表明,对叶百部碱的标准曲线为Y=0.003 7X+0.089(R2=0.995 9),在32~800μg/mL范围内线性关系良好;平均加样回收率为101.23%,平均加样回收率的RSD为2.63%。1年生对叶百部总生物碱含量为1.87~4.55 mg/g;2年生对叶百部总生物碱含量为4.52~9.63 mg/g。酸性染料比色法具有操作简便、重复性好、稳定性高的优点。The total alkaloid content in Stemona tuberosa Lour.at different developmental stages was determined using the acid dye colorimetric method.The acid dye colorimetric method was used,with Stemona tuberosa Lour.alkali as the reference material and bro⁃mothymol blue as the acid dye.Methanol and chloroform were extracted,and dissolved in 4%hydrochloric acid.The determination was carried out at a wavelength of 413 nm.The results showed that the standard curve of Stemona tuberosa Lour.alkali was Y=0.0037X+0.089(R2=0.9959).The linear relationship was good from 32 to 800μg/mL;the average sample recovery rate was 101.23%,and the RSD of the average sample recovery rate was 2.63%.The total alkaloid content of annual Stemona tuberosa Lour.was 1.87~4.55 mg/g;the total alkaloid content of the biennial Stemona tuberosa Lour.was 4.52~9.63 mg/g.The acid dye colorimetric method had the advan⁃tages of simple operation,good repeatability,and high stability.

关 键 词：对叶百部(Stemona tuberosa Lour.) 总生物碱 酸性染料比色法 不同发育时期 

分 类 号：R917[医药卫生—药物分析学]