健脾化浊调脂颗粒的化学成分及入血成分分析  

Identification of chemical components and components migrating to the blood of Jianpi huazhuo tiaozhi granules

作　　者：王逸然 刘中勇[2] WANG Yiran;LIU Zhongyong(School of Clinical Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Dept.of Cardiovascular Medicine,the Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330006,China)

机构地区：[1]江西中医药大学临床医学院,南昌330004 [2]江西中医药大学附属医院心血管科,南昌330006

出　　处：《中国药房》2024年第2期140-144,共5页China Pharmacy

基　　金：国家自然科学基金项目(No.81960849);国家中医药管理局高水平中医药重点学科建设项目(No.zyyzdxk-2023113);全国名老中医药专家传承工作室建设项目(No.国中医药人教函[2022]75号);江西中医药大学校级研究生创新专项资金立项资助项目(No.JZYC23S50)。

摘　　要：目的分析健脾化浊调脂颗粒(JHTG)的化学成分及其入血成分。方法将SD大鼠分为对照组和给药组,每组6只。给药组大鼠灌胃JHTG药液3 mL。灌胃60 min后,收集两组大鼠血清样品,采用超高效液相色谱-串联质谱技术对JHTG的化学成分及入血成分进行色谱分离和质谱数据采集,结合UNIFI天然产物整体解决方案,基于6400天然产物理论质谱数据库并通过文献查阅、对照品比对等进行结构分析、确认。结果与结论从JHTG中共鉴定出130个成分,其中党参3个、荷叶13个、茯苓15个、白术5个、陈皮9个、薏苡仁1个、泽泻19个、丹参24个、麦芽7个、山楂24个、砂仁2个、木香3个,另外槲皮素、槲皮素-3-O-β-D-吡喃葡萄糖苷、山柰黄素、柠檬酸可能来源于荷叶或山楂,土当归酸可能来源于茯苓或山楂;通过与对照品比对,最终确定了8个成分(茯苓酸、白术内酯Ⅱ、泽泻醇A、泽泻醇B、土木香内酯、乙酸龙脑酯、丹酚酸A、丹酚酸C)。共鉴定出槲皮素、山柰黄素等72个入血原型成分,以黄酮类、萜类、木脂素类、酚酸类成分为主。共鉴定出去氢番荔枝碱、16-氧乙酰茯苓酸等11个代谢产物,以萜类成分为主;代谢途径包括脱氢、脱羟基等Ⅰ相代谢反应及甲基化、乙酰化等Ⅱ相代谢反应。OBJECTIVE To analyze the chemical components and components migrating to the blood of Jianpi huazhuo tiaozhi granules(JHTG).METHODS SD rats were divided into a control group and a medication group,with 6 rats in each group.The medication group was given JHTG 3 mL.Sixty minutes after medication,the serum samples of the 2 groups were collected,and the chemical components and components migrating to the blood of JHTG were separated by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and mass spectrometry data were collected.Combined with the overall scheme of UNIFI natural products,based on the 6400 natural product theory mass spectrometry database,the structure was analyzed and confirmed by literature review and reference substance comparison.RESULTS&CONCLUSIONS A total of 130 components were identified from JHTG,including 3 in Codonopsis Radix,13 in Nelumbinis Folium,15 in Poria,5 in Atractylodis Macrocephalae Rhizoma,9 in Citri Reticulatae Pericarpium,1 in Coicis Semen,19 in Alisma Rhizoma,24 in Salviae Miltiorrhizae Radix et Rhizoma,7 in Hordei Fructus Germinatus,24 in Crataegi Fructus,2 in Amomi Fructus,and 3 in Aucklandiae Radix.In addition,quercetin and quercetin-3-O-β-D-glucopyranoside,kaempferol and citric acid may originate from Nelumbinis Folium or Crataegi Fructus,while oleanolic acid may originate from Poria or Crataegi Fructus.By comparing the reference substances,8 components were finally determined(pachymic acid,atractylenolideⅡ,alisol A,alisol B,alantolactone,bornyl acetate,salvianolic acid A,salvianolic acid C).A total of 72 prototype components such as quercetin and kaempferol were identified,mainly including flavonoids,terpenoids,lignans and phenolic acids.A total of 11 metabolites such as dehydroanonaine and 16-O-acetylpachymic acid were identified,mainly terpenoids.Metabolic pathways include phaseⅠmetabolic reactions such as dehydrogenation and dehydroxylation,and phaseⅡmetabolic reactions such as methylation and acetylation.

关 键 词：健脾化浊调脂颗粒 化学成分 入血成分 超高效液相色谱-串联质谱技术 

分 类 号：R917[医药卫生—药物分析学] R284.1[医药卫生—药学]