缺血缺氧模型小鼠少突胶质前体细胞GluR2磷酸化水平的变化  

The changing levels of GluR2 phosphorylation in oligodendrocyteprecursor cells in mice model of ischaemic hypoxia

作　　者：甄飞 姚瑞芹 邱振 ZHEN Fei;YAO Ruiqin;QIU Zhen(Laboratory of Pathology,Hongze District People’s Hospital,Huai’an 223110;Department of Cell Biology and Neurobiology,Xuzhou Medical University,Xuzhou 221004,China)

机构地区：[1]淮安市洪泽区人民医院病理科,淮安223110 [2]徐州医科大学细胞生物与神经生物学实验室,徐州221004

出　　处：《神经解剖学杂志》2023年第6期671-676,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金项目(81771337)。

摘　　要：目的:研究缺血缺氧性脑损伤(HIBI)模型小鼠少突胶质前体细胞(OPCs)中磷酸化谷氨酸受体2[p-GluR2(S880)]表达变化。方法:利用右侧颈总动脉结扎并缺氧90 min方法建立C57BL/6新生小鼠HIBI模型,采用高架十字迷宫和旷场实验评估小鼠的焦虑样行为,通过免疫荧光方法检测HIBI模型小鼠脑组织中p-GluR2(S880)、少突胶质细胞标记物4(O4)和髓鞘碱性蛋白(MBP)的表达变化,用Western Blot检测p-GluR2(S880)和MBP的表达水平。结果:与假手术组相比,HIBI术后90 d小鼠有显著的焦虑样行为(P<0.05);MBP蛋白表达在HIBI术后14及28 d组明显减少;p-GluR2(S880)蛋白表达在HIBI术后各个时间点表达上调(P<0.05),HIBI组小鼠脑组织中O4与p-GluR2(S880)共标的阳性细胞数目显著升高(P<0.05)。结论:OPCs中p-GluR2(S880)表达上调可能导致HIBI模型小鼠成髓鞘障碍。Objective:To investigate the expression of phosphorylated glutamate receptor 2[p-GluR2(S880)]in oligodendrocyte precursor cells(OPCs)of mice model of hypoxic ischemic brain injury(HIBI).Methods:The HIBI model of C57BL/6 neonatal mice was established by right common carotid artery ligation and hypoxia for 90 min.The anxiety-like behavior of the mice was evaluated by elevated plus maze(EPM)and open field test(OFT).The expression of p-GluR2(S880),oligodendrocyte marker 4(O4)and myelin basic protein(MBP)in brain tissue of HIBI model mice was detected by immunofluorescence staining.What’s more,the expression levels of p-GluR2(S880)and MBP were detected by Western Blot.Results:Compared with sham operation group,there were significant anxiety-like behaviors 90 days after HIBI operation(P<0.05).The expression of MBP protein decreased significantly in 14 and 28 days after HIBI operation.The expression of p-GluR2(S880)protein was up-regulated at all time points after HIBI operation(P<0.05),and the number of O4 and p-GluR2(S880)double positive cells in brain tissue of HIBI group was significantly increased(P<0.05).Conclusion:The up-regulation of p-GluR2(S880)expression in OPCs may lead to myelination disorder in HIBI model mice.

关 键 词：谷氨酸受体2 缺血缺氧 脑白质损伤 少突胶质前体细胞 小鼠 

分 类 号：R722.6[医药卫生—儿科] R-332[医药卫生—临床医学]