蒙古黄芪茎叶多类型资源性化学成分分析与价值评价  

Chemical composition analysis and value evaluation of stems and leaves of Astragalus membranaceus var.mongholicus

作　　者：王强雄 郭盛[1] 申柯欣 李会伟 张浩宽 谢逸俊 尚尔鑫[1] 段金廒[1] WANG Qiang-xiong;GUO Sheng;SHEN Ke-xin;LI Hui-wei;ZHANG Hao-kuan;XIE Yi-jun;SHANG Er-xin;DUAN Jin-ao(National Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学,中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏省中药资源产业化过程协同创新中心,国家中医药管理局中药资源循环利用重点研究室,江苏南京210023

出　　处：《中国中药杂志》2023年第24期6600-6612,共13页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202005);中央本级重大增减支项目(2060302);宁夏回族自治区重点研发计划项目(2017BY079)。

摘　　要：通过分析和评价蒙古黄芪茎叶多类型资源性化学成分,为其资源化利用提供数据支撑。分别采用凯氏定氮法、过滤法、紫外-可见分光光度法对蒙古黄芪茎叶中粗蛋白、粗纤维、可溶性糖类成分进行分析;采用超高效液相色谱-三重四极杆质谱串联(UPLC-TQ-MS)法对蒙古黄芪茎叶核苷类、氨基酸类、黄酮类及皂苷类成分进行分析。结合主成分分析对蒙古黄芪茎叶资源性成分质量差异进行综合评价。结果表明蒙古黄芪茎叶中粗蛋白、粗纤维、总多糖、还原糖平均质量分数分别为5.11%、30.33%、11.03 mg·g^(-1)、31.90 mg·g^(-1);检测出6种核苷类成分、15种氨基酸类成分、22种黄酮类成分和1种皂苷类成分,平均总量分别为1.49 mg·g^(-1)、6.00 mg·g^(-1)、1.86 mg·g^(-1)、35.67μg·g^(-1);不同采收期、不同生长年限蒙古黄芪茎叶各类型资源性化学成分含量差异显著;主成分分析结果表明宁夏产蒙古黄芪茎叶质量较优。研究结果可为蒙古黄芪茎叶资源化利用提供参考和依据。This study aimed to provide data support for resource utilization of the stems and leaves of Astragalus membranaceus var.mongholicus(SLAM)by analyzing and evaluating the chemical constituents.The crude protein,crude fiber,and soluble saccharide of SLAM were analyzed by Kjeldahl method,filtration method,and UV-Vis spectrophotometry,respectively.The nucleosides,amino acids,flavonoids,and saponins of SLAM were analyzed by ultraperformance liquid chromatography-triple quadrupole mass spectrometry(UPLCTQ-MS).Combined with principal component analysis(PCA),the quality difference of resource components of SLAM was comprehensively evaluated.The results showed that the average content of crude protein,crude fiber,total polysaccharide,and redu-cing sugar in SLAM was 5.11%,30.33%,11.03 mg·g^(-1),and 31.90 mg·g^(-1),respectively.Six nucleosides,15 amino acids,22 flavonoids,and one saponin were detected,with an average content of 1.49 mg·g^(-1),6.00 mg·g^(-1),1.86 mg·g^(-1),and 35.67μg·g^(-1),respectively.The content of various types of chemical components in SLAM differed greatly in different harvesting periods and growing years.The results of PCA showed that the quality of SLAM produced in Ningxia was superior.The results can provide references for the utilization of SLAM.

关 键 词：黄芪 蒙古黄芪茎叶 资源化学 品质分析 资源化利用 

分 类 号：R284.1[医药卫生—中药学]