响应面法优化壳聚糖对人参皂苷提取液的除杂工艺  

Response Surface Method to Optimize Impurity Removal Process of Chitosan to Ginsenoside Extract

作　　者：罗芳 范贤哲 吕敬崑 张存根 宋佳 陈小明 LUO Fang;FAN Xian-zhe;LU Jing-kun;ZHANG Cun-gen;SONG Jia;CHEN Xiao-ming(School of Chemistry and Engineering,Hunan University of Science and Engineering,Hunan Yongzhou 425100;Hunan Hengwei Yaoye Co.,Ltd.,Hunan Yongzhou 425100,China)

机构地区：[1]湖南科技学院化学与生物工程学院,湖南永州425100 [2]湖南恒伟药业股份有限公司,湖南永州425100

出　　处：《广州化工》2023年第17期44-47,53,共5页GuangZhou Chemical Industry

基　　金：湖南省自然科学基金项目(项目编号:2020JJ9026)。

摘　　要：利用响应面分析方法优化壳聚糖对人参皂苷提取液的除杂工艺,以壳聚糖用量、作用温度和搅拌时间为响应因素,综合评分(OD)为响应值,实施三因素三水平的响应面分析,建立响应面模型,并得出最佳工艺条件。结果表明,人参皂苷提取液除杂最佳工艺为:壳聚糖用量(提取液与壳聚糖溶液体积比)1.2∶1,作用温度70℃,搅拌时间100 min。人参皂苷提取液除杂工艺优化后,综合评分(OD)为94.43%,RSD为0.45%,重现性较好,表明该模型有效。The response surface analysis method was used to optimize the impurity removal process of chitosan to ginsenoside extract,and the response surface analysis of three factors and three levels was carried out with chitosan dosage,action temperature and stirring time as the response factors,and the comprehensive score(OD)was used as the response value,and the response surface model was established,and the optimal process conditions were obtained.The results showed that the optimal process for removing impurities from ginsenoside extract was as follows:chitosan dosage(volume ratio between extract and chitosan solution)was 1.21,action temperature was 70℃,stirring time was 100 min.After the optimization of the impurity removal process of ginsenoside extract,the comprehensive score(OD)was 94.43%and RSD was 0.45%,which showed good reproducibility,indicating that the model was effective.

关 键 词：壳聚糖 人参皂苷 响应面法 除杂工艺 

分 类 号：R943.1[医药卫生—药剂学]