一株褐家鼠戊型肝炎病毒的发现鉴定及系统发生分析  

Identification and Phylogenetic Analysis of a Strain of Hepatitis E Virus from Rattus norvegicus

作　　者：宗利东 殷红敏 杨银[2] 赵维玉 徐粉慧 韩培钰 崔凤灵 何明仙 张云智 ZONG Lidong;YIN Hongmin;YANG Yin;ZHAO Weiyu;XU Fenhui;HAN Peiyu;CUI Fengling;HE Mingxian;ZHANG Yunzhi(School of Public Health,Institute of Preventive Medicine,Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan,Key Laboratory for Cross-Border Control and Quarantine of Zoonoses in Universities of Yunnan Province,Dali University,Dali 671000,China;The Second People’s Hospital of Dali Prefecture,Dali 671000,China)

机构地区：[1]大理大学公共卫生学院,大理大学预防医学研究所,云南省滇西抗病原植物资源筛选研究重点实验室,云南省高校人兽共患病跨境防控与检疫重点实验室,大理671000 [2]大理州第二人民医院,大理671000

出　　处：《激光生物学报》2023年第6期548-553,560,共7页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(U2002218,81874274);云南省高层次卫生计生技术人才培养项目(L-2017027);大理大学博士科研启动费项目(KYBS2018004);大理大学人兽共患病跨境防控与检疫创新团体项目(ZKPY2019302)。

摘　　要：对云南省大理市小型哺乳动物携带的戊型肝炎病毒(HEV)进行调查,用巢式RT-PCR对捕获的小型哺乳动物样品中的病毒进行检测,用高通量测序对阳性HEV样本进行毒株全基因组测序,用MEGA-X、BESTv1.10.4和Geneious软件进行生物信息学分析。本研究筛查了6属7种共114只小型哺乳动物样本,结果显示:HEV在褐家鼠中的阳性率为8.00%(2/25);高通量测序获得1株编号为DL147的HEV全基因组,经系统发生分析确定其为HEV-C1基因型,与香港1例戊型肝炎患者血清中发现的HEV同源性最高,核苷酸序列一致性达95.21%,两株病毒的最早分歧时间约为2003年。研究证实,云南省大理市褐家鼠存在HEV-C1感染,其与近期报道的人源新发HEV高度同源,有较近的系统发生关系,对戊型肝炎防控有指导作用。Hepatitis E virus(HEV)harbored by small mammals in Dali City,Yunnan Province was investigated.The samples were detected by nested RT-PCR and whole genomes were sequenced by high-throughput sequencing in the positive HEV sam-ples.Bioinformatics was analyzed by the soft-wares of MEGA-X,BESTv1.10.4 and Geneious.A total of 114 small mammals from 6 genera and 7 species were screened in this study.The positivity rate of HEV in Norway rat(Rattus norvegicus)was 8.00%(2/25),and the whole genome of one HEV strain of No.DL147 was obtained,which was the HEV-C1 genotype,and had the highest homology to HEV in the serum of a patient with hepatitis E in Hong Kong,with a nucleotide level of 95.21%.The earliest divergence between the two strains was about 2003.The study confirmed the presence of HEV-C1 infection in Norway rat in Dali City,Yunnan Province,which is highly homologous to the recently reported human emerging HEV and has a relatively recent phylogenetic relationship,and it is instructive for prevention and control of hepatitis E.

关 键 词：戊型肝炎病毒 人兽共患病 啮齿动物 遗传分析 高通量测序 

分 类 号：R183.4[医药卫生—流行病学]