基于间接波前整形的近红外二区荧光共聚焦成像研究  

NIR-II fluorescence confocal imaging based on indirect wavefront shaping

作　　者：谭天 史天悦 吴长锋 彭洪尚[1] TAN Tian;SHI Tian-yue;WU Chang-feng;PENG Hong-shang(Engineering Research Center of Photonic Design Software Ministry of Education,College of Science,Minzu University of China,Beijing 100081,China;Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen 518055,China)

机构地区：[1]中央民族大学理学院光子系统工程软件教育部工程研究中心,北京100081 [2]南方科技大学生物医学工程系,广东深圳518055

出　　处：《中国光学（中英文）》2024年第1期150-159,共10页Chinese Optics

基　　金：国家自然科学基金(No.62175266,No.62235007,No.22204070);深圳市科技计划项目(No.KQTD20170810111314625,No.JCYJ20210324115807021,No.SGDX20211123114002003);深圳湾实验室开放课题(No.SZBL2021080601002);广东省先进生物材料重点实验室(No.2022B1212010003)。

摘　　要：生物组织散射引起的光学像差限制了光学系统的成像性能。本文研究了基于间接波前整形的近红外二区荧光共聚焦成像技术。首先,制备了高效率近红外二区荧光探针,降低该波段生物组织的散射有助于实现高对比度的活体组织成像。其次,研究了基于间接波前测量的自适应光学方法,将间接波前整形技术应用于激光扫描共聚焦显微系统中,以实现对生物组织引起的光学像差的测量与补偿,获得生物组织的高信噪比成像。最后,对基于间接波前整形的近红外二区荧光共聚焦成像系统开展了相关实验。实验结果表明,本系统对空气平板、散射介质和小鼠颅骨等产生的像差具有良好的补偿效果,最终信号强度较初始值分别提升了1.47、1.95和2.85倍,显著提升了最终的成像质量。Optical aberrations caused by the scattering of biological tissues limit the imaging performance of optical systems.A near-infrared II fluorescence confocal imaging technique based on indirect wavefront shaping was investigated.First,we synthesized a highly efficient near-infrared II range fluorescent probe,where reducing the scattering of biological tissue can realize biopsy imaging with high-contrast.Second,we investigated the adaptive optical method based on indirect wavefront measurement.The indirect wavefront shaping technology was applied to the laser scanning confocal system,enabling the measurement and com-pensation of optical aberrations caused by biological tissues,and obtaining imaging of biological tissues with a high signal-to-noise ratio.Finally,near-infrared II fluorescence confocal imaging system based on indirect wavefront shaping was deployed and relevant experiments were conducted.The experimental results indic-ate that the system effectively compensates for the aberrations induced by air plates,scattering media and mouse skull,and increases the final signal intensity by 1.47,1.95 and 2.85 times,respectively.As a result,the final imaging quality is significantly enhanced.

关 键 词：间接波前整形 近红外二区成像 共聚焦成像 活体实验 

分 类 号：O439[机械工程—光学工程]