红螯螯虾源布氏柠檬酸杆菌分离鉴定和耐药性分析  

Isolation,Identification and Drug Resistance Analysis of Citrobacter braakii from Cherax quadricarinatus

作　　者：黄亚明 李长乐 覃诗君 陆唯 张博洲 孙佳艺 张文旗 童潼 张琴 HUANG Yaming;LI Changle;QIN Shijun;LU Wei;ZHANG Bozhou;SUN Jiayi;ZHANG Wenqi;TONG Tong;ZHANG Qin(Key Laboratory of Protection and Utilization of Marine Resources,Guangxi Laboratory of Polysaccharide Materials and Modification,School of Biotechnology and Marine Science,Guangxi Minzu University,Nanning 530006,China)

机构地区：[1]广西民族大学海洋与生物技术学院广西多糖材料与改性重点实验室广西海洋微生物资源产业化工程技术研究中心,广西南宁530006

出　　处：《中国兽医杂志》2024年第1期59-65,共7页Chinese Journal of Veterinary Medicine

基　　金：广西民族大学引进人才科研启动项目(2018KJQD14);广西创新驱动发展专项资金项目(桂科AA17204044);广西民族大学相思湖青年学者创新团队资助项目(2018RSCXSHQN02);大学生创新训练项目(S202210608159)。

摘　　要：为了确定广西壮族自治区吴圩镇某养殖场红螯螯虾(Cherax quadricarinatus)的潜在致病菌,本试验于2021年1月从该场螯虾肠道分离获得1株优势菌,命名为171215-10,采用形态学观察、生化鉴定、基于16S rDNA序列构建系统进化树、菌株生长曲线测定、人工感染斑马鱼和Kirby-Bauer(K-B)纸片扩散法对分离菌株进行鉴定和分析。结果显示,经鉴定分离菌株为布氏柠檬酸杆菌(Citrobacter braakii),在5~16 h处于指数生长期。人工感染斑马鱼试验结果显示,分离菌株具有较强致病性,注射浓度为1.5×10^(8)CFU/mL布氏柠檬酸杆菌的斑马鱼在2 d内全部死亡。药敏试验结果显示,分离菌株仅对25种抗菌药中的5种敏感,包括诺氟沙星(NFX)、氯霉素(CLM)、多西环素(DOX)、阿米卡星(AMK)和庆大霉素(GEN)。本试验结果为红螯螯虾源布氏柠檬酸杆菌的鉴定及其诊断和治疗提供参考。In order to identify potential pathogens in Cherax quadricarinatus from a farm in Wuxu Town,Guangxi Zhuang Autonomous Region,China,a dominant strain named 171215-10 was isolated from the crayfish intestines in January 2021.Identification and analysis of the isolated strain were conducted using morphological observation,biochemical identification,construction of phylogenetic tree based on 16S rDNA sequence,bacterial growth curve determination,artificial infection of zebrafish,and Kirby-Bauer(K-B)disk diffusion method.The results showed that the identified isolated strain was Citrobacter braakii,which exhibited exponential growth from 5 to 16 hours.The artificial infection experiment on zebrafish demonstrated that the isolated strain was highly pathogenic,zebrafish injected with a concentration of 1.5×10^(8) CFU/mL of Citrobacter braakii died within 2 days.Drug sensitivity testing revealed that the isolated strain was sensitive to only 5 out of 25 antibacterial drugs,including norfloxacin(NFX),chloramphenicol(CLM),doxycycline(DOX),amikacin(AMK),and gentamicin(GEN).The results of this study provide reference information for the identification,diagnosis,and treatment of Citrobacter braakii from Cherax quadricarinatus.

关 键 词：布氏柠檬酸杆菌 红螯螯虾 分离鉴定 人工感染试验 药敏试验 

分 类 号：R378.2[医药卫生—病原生物学]