电针“天枢”与“天枢”配伍“上巨虚”对肠易激综合征大鼠行为学和结肠功能的影响  

Effect of Electroacupuncture with"Tianshu"and"Tianshu"Combined with"Shangjuxu"on Behavior and Colon Function in Rats with Irritable Bowel Syndrome

作　　者：刘婷婷[1] 张擎宇 赵香顺 石运来 于燕南 王正文 陈少宗[4] 杨添淞[1,5,6] LIU Tingting;Zhang Qingyu;ZHAO Xiangshun;SHI Yunlai;YU Yannan;WANG Zhengwen;CHEN Shaozong;YANG Tiansong(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Ji'nan Hospital,Ji'nan 250013 China;College of Acupuncture and Moxibustion and Massage,Shandong University of Traditional Chinese Medicine,Ji'nan 250355,China;Institute of Acupuncture and Moxibustion in Shandong University of Traditional Chinese,Ji'nan 250355,China;The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Key Laboratory of Chinese Medicine Informotics in Heilongjiang Province,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]济南医院,济南250013 [3]山东中医药大学针灸推拿学院,济南250355 [4]山东中医药大学针灸研究院,济南250355 [5]黑龙江中医药大学附属第一医院,哈尔滨150040 [6]黑龙江省中医药信息学重点实验室,哈尔滨150040

出　　处：《中国中医基础医学杂志》2024年第2期245-250,共6页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家重点研发计划(2019YFC1712100)。

摘　　要：目的观察并比较电针“天枢”与“天枢”配伍“上巨虚”对肠易激综合征(irritable bowel syndrome,IBS)模型大鼠情绪和结肠功能影响,初步探讨天枢配伍上巨虚是否存在协同或叠加效应。方法雄性Wistar大鼠分为空白组(10只)和造模组(45只),采用慢性和急性应激相结合方法连续干预10 d制备模型,将制备成功IBS模型大鼠随机分为模型组(8只)、天枢组(8只)、天枢配伍上巨虚组(8只);天枢组、天枢配伍上巨虚组连续电针干预治疗14 d;干预结束后,分别对各组大鼠进行高架十字迷宫实验、结肠痛敏阈值测定,检测血清5-羟色胺(5-hydroxytryptamine,5-HT)含量以及结肠5-羟色胺4受体(5-hydroxytryptamine 4 receptor,5-HT 4 R)蛋白表达。结果与模型组比较,电针天枢组、电针天枢配伍上巨虚组进入开放臂(open arm entry,OE)次数百分比和开臂停留时间(open arm time,OT)百分比增加(P<0.01);结肠痛敏阈值显著升高(P<0.01);血清5-HT含量减少(P<0.05);结肠5-HT 4 R蛋白相对表达量增加(P<0.05)。两电针组之间比较,OE%、OT%、结肠痛敏阈值、血清5-HT含量以及结肠5-HT 4 R蛋白相对表达量的差异无统计学意义。结论电针“天枢”与“天枢”配伍“上巨虚”均能调节大鼠情绪心理行为的异常和改善结肠功能紊乱症状,但电针“天枢”配伍“上巨虚”相较于电针“天枢”未表现明显的穴位协同或叠加效应。Objective To observe and compare the effects of electroacupuncture"Tianshu"(ST25)and"Tianshu"combined with"Shangjuxu"(ST37)on the mood and colon function of rats with irritable bowel syndrome(IBS)model.To observe and compare the effects of EA-ST25,EA-ST25+ST37 on the mood and colon function of rats modeling IBS,and to preliminarily investigate whether there is any synergistic or superimposed effect of ST25 and ST37.Methods 55 male clean-grade Wistar rats,were randomly divided into Control(10 rats),model groups(45 rats).The model rats were intervened for 10 days by the combination of chronic and acute stress;After the end of modeling,male Wistar rats were randomly divided into model(8 rats),EA-ST25(8 rats),EA-ST25+ST37 groups(8 rats).The two treatment groups received continuous EA interventions for 14 days,and at the end of the intervention,each group of rats was subjected to the elevated cross-maze test,the determination of colonic pain threshold,the measurement of serum 5-hydroxytryptamine(5-HT)and the expression of 5-hydroxytryptamine 4 receptor(5-HT 4R)protein in the colon.Results Compared with the model group,the percentage of entries into the open arm and time increased and were statistically significant(P<0.01)in the EA-ST25 and the EA-ST25+ST37.The visceral pain sensitivity thresholds of the ST25 and the ST25+ST37 were significantly higher and statistically significant(P<0.01).The serum 5-HT contents of the EA-ST25 and the EA-ST25+ST37 decreased(P<0.05).The relative 5-HT 4 R receptor protein expression in the EA-ST25 or EA-ST25+ST37 increased(P<0.05);There was no statistically significant difference between the two treatment groups.Conclusion EA-ST25、ST25+ST37 group can both regulate the abnormalities of emotional and psychological behavior and improve the symptoms of disordered colon function in rats,but compared with EA-ST25,EA-ST25+ST37 did not show a significant synergistic effect.

关 键 词：肠易激综合征 天枢配伍上巨虚 高架十字迷宫 结肠功能 

分 类 号：R245[医药卫生—针灸推拿学]