Co-expression of GR79 EPSPS and GAT generates highglyphosate-resistant alfalfa with low glyphosate residues  

作　　者：Yingying Meng Wenwen Zhang Zhaoming Wang Feng Yuan Sandui Guo Hao Lin Lifang Niu 

机构地区：[1]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Inner Mongolia Pratacultural Technology Innovation Center Co.,Ltd,Hohhot 010010,China [3]National Center of Pratacultural Technology Innovation(Under Preparation),Hohhot 010010,China

出　　处：《aBIOTECH》2023年第4期352-358,共7页生物技术通报（英文版）

基　　金：supported by grants from Ministry of Science and Technology of the People’s Republic of China(2022YFF1003204);National Natural Science Foundation of China(32325035);Hainan Yazhou Bay Seed Laboratory(B21HJ0215),Hohhot Key R&D Project(2023-JBGS-S-1);National Center of Pratacultural Technology Innovation(under preparation)(CCPTZX2023B01).

摘　　要：Weed competition seriously threatens the yield of alfalfa, the most important forage legume worldwide,thus generating herbicide-resistant alfalfa varieties is becoming a necessary cost-effective strategy toassist farmers for weed control. Here, we report the co-expression of plant codon-optimized forms ofGR79 EPSPS (pGR79 EPSPS) and N-acetyltransferase (pGAT) genes, in alfalfa, via Agrobacterium-mediated transformation. We established that the pGR79 EPSPS-pGAT co-expression alfalfa lines were able totolerate up to tenfold higher commercial usage of glyphosate and produced approximately ten timeslower glyphosate residues than the conventional cultivar. Our findings generate an elite herbicideresistant germplasm for alfalfa breeding and provide a promising strategy for developing high-glyphosate-resistant and low-glyphosate-residue forages.

关 键 词：ALFALFA pGR79 EPSPS pGAT High-glyphosate-resistant and low-glyphosate-residue Forage 

分 类 号：S54[农业科学—作物学]