Lipidomic insights into the response of Arabidopsis sepals to mild heat  

作　　者：Zican Chen Weronika Jasinska Muhammad Ashraf Leah Rosental Jung Hong Dabing Zhang Yariv Brotman Jianxin Shi 

机构地区：[1]Joint International Research Laboratory of Metabolic and Developmental Sciences,State Key Laboratory of Hybrid Rice,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China [2]Department of Life Sciences,Ben Gurion University of the Negev,Beersheva 84105,Israel [3]School of Agriculture,Food and Wine,University of Adelaide,Urrbrae,SA 5064,Australia [4]Yazhou Bay Institute of Deepsea Sci-Tech,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《aBIOTECH》2023年第3期224-237,共14页生物技术通报（英文版）

基　　金：supported by National Natural Sciences Foundation of China(31671511 and 31971907);the SJTU JiRLMDS Joint Research Fund(MDS-JF-2019B02);the Programme of Introducing Talents of Discipline to Universities(111 Project,B14016).

摘　　要：Arabidopsis sepals coordinate flower opening in the morning as ambient temperature rises;however,the underlying molecular mechanisms are poorly understood. Mutation of one heat shock proteinencoding gene, HSP70-16, impaired sepal heat stress responses (HSR), disrupting lipid metabolism,especially sepal cuticular lipids, leading to abnormal flower opening. To further explore, to what extent,lipids play roles in this process, in this study, we compared lipidomic changes in sepals of hsp70-16 andvdac3 (mutant of a voltage-dependent anion channel, VDAC3, an HSP70-16 interactor) grown underboth normal (22 C) and mild heat stress (27 C, mild HS) temperatures. Under normal temperature,neither hsp70-16 nor vdac3 sepals showed significant changes in total lipids;however, vdac3 but nothsp70-16 sepals exhibited significant reductions in the ratios of all detected 11 lipid classes, except themonogalactosyldiacylglycerols (MGDGs). Under mild HS temperature, hsp70-16 but not vdac3 sepalsshowed dramatic reduction in total lipids. In addition, vdac3 sepals exhibited a significant accumulationof plastidic lipids, especially sulfoquinovosyldiacylglycerols (SQDGs) and phosphatidylglycerols (PGs),whereas hsp70-16 sepals had a significant accumulation of triacylglycerols (TAGs) and simultaneousdramatic reductions in SQDGs and phospholipids (PLs), such as phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), and phosphatidylserines (PSs). These findings revealed that the impact ofmild HS on sepal lipidome is influenced by genetic factors, and further, that HSP70-16 and VDAC3differently affect sepal lipidomic responses to mild HS. Our studies provide a lipidomic insight intofunctions of HSP and VDAC proteins in the plant’s HSR, in the context of floral development.

关 键 词：Floral development Heat shock protein Lipidomic profiling Stress response Voltage-dependent anion channel protein 

分 类 号：Q94[生物学—植物学]