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作 者:付深造 任君 王鑫 王宁宁 房建 徐东辉[1] 杨坤 FU Shenzao;REN Jun;WANG Xin;WANG Ningning;FANG Jian;XU Donghui;YANG Kun(State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
机构地区:[1]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081
出 处:《园艺学报》2023年第12期2665-2679,共15页Acta Horticulturae Sinica
基 金:农业农村部物种品种资源保护费项目(999900001046)。
摘 要:以华北型、华南型和欧洲型黄瓜各1个品种为接穗,以1个南瓜品种为砧木嫁接,依据《NY/T2235-2012植物新品种特异性、一致性和稳定性测试指南黄瓜》和UPOV测试指南TG/61/7探讨嫁接对其50个DUS测试基本性状和5个果实品质指标的影响。结果表明:(1)50个DUS测试基本性状中的10个质量性状、7个假质量性状和7个目测数量性状的代码在3个品种嫁接处理和自根对照间保持不变,性状稳定性好,可作为砧木嫁接黄瓜品种鉴别的主要性状;6个目测数量性状在嫁接处理和自根对照间代码值相差1,差异不显著,可作为砧木嫁接黄瓜品种鉴别的备选性状;而砧木嫁接对个体测量数量性状有影响,其中主蔓节间长度、叶片长度在3个品种嫁接处理和自根对照间的差异达极显著水平,不宜用于砧木嫁接黄瓜品种的鉴别;(2)与自根对照相比,3个品种嫁接处理果实蛋白质含量均显著提高,可溶性固形物含量、维生素C含量、可溶性糖含量没有显著变化,华北型品种嫁接处理中干物质含量极显著降低,而华南型和欧洲型品种没有显著变化。Using one variety each for North China type,South China type,and European type as scion,and one pumpkin variety as rootstock,the effects of rootstock grafting on the expression of 50 basic DUS characteristics and five fruit quality indicators of cucumber varieties were studied in accordance with the“NY/T 2235-2012 guidelines for the conduct of tests for distinctness,uniformity and stability-cucumber”and UPOV Testing Guidelines TG/61/7.The results showed that:(1)Among the 50 basic DUS characteristics,ten qualitative characteristics,seven pseudo-qualitative characteristics,and seven quantitative characteristics which were visually assessed remained unchanged between the grafted and self-rooted treatments of the three varieties,indicated that these characteristics had good stability and they could be used as the main characteristics for identifying cucumber varieties grafted with rootstocks;the notes of other six quantitative characteristics which were visually assessed differed by one between the grafted and self-rooted treatments,resulting in the difference was not significant,so these characteristics could be used as the alternative characteristics for identifying cucumber varieties grafted with rootstocks.While,grafting had an impact on the quantitative characteristics which were measured by individuals,with significant differences in internode length of main vine and length of leaf blade between the grafted and self-rooted treatments of the three varieties,making them unsuitable for identifying cucumber varieties grafted with rootstocks.(2)Compared with the self-rooted plant,the protein content of the fruits in the grafted plant of three varieties was significantly increased,while the soluble solid content,vitamin C content and soluble sugar content did not significantly change.The dry matter content in the grafted plant of North China type variety was significantly reduced,while there was no significant change in the South China and European type varieties.
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