纽荷尔脐橙内生真菌参与生理落果的研究  

Endophytic Fungi and the Relations to the Fruit Drop of Citrus

作　　者：崔雪婧 张家宁 陈江华 贾纪春 林杨 付艳苹[1,2] CUI Xuejing;ZHANG Jianing;CHEN Jianghua;JIA Jichun;LIN Yang;FU Yanping(Key Laboratory of Plant Pathology of Hubei Province,College of Plant Science&Technology,Huazhong Agricultural University,Wuhan 430070,China;National R&D Center for Citrus Postharvest Technology,Wuhan 430070,China;College of Plant Protection,Shanxi Agricultural University,Taigu,Shanxi 030801,China)

机构地区：[1]华中农业大学植物科学技术学院,湖北省作物病害检测和安全控制重点实验室,武汉430070 [2]国家柑橘保鲜技术研发专业中心,武汉430070 [3]山西农业大学植物保护学院,山西太谷030801

出　　处：《园艺学报》2023年第12期2713-2722,共10页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2021YFD1600103);云南省重大科技专项计划项目(202102AE090105);现代农业产业技术体系建设专项(CARS-26)。

摘　　要：采用ITS高通量测序技术结合真菌分离和培养的方法,研究了纽荷尔脐橙第2次生理落果期间黄化即将脱落的和健康的果实内真菌的多样性,发现即将脱落的果实真菌群落的丰富度、均匀度、物种数显著高于健康果实,并且真菌群落的组成结构也存在显著差异。通过组织分离方法获得了黄化即将脱落的果实中的真菌菌株23株,采用刺伤接种的方法将部分菌株接种于纽荷尔脐橙花后50 d的果实,发现均能引起果实黄化,其中镰刀菌属菌株能够引起幼果脱落酸含量和乙烯释放速率升高。研究结果提示内生真菌群落及引起的内源激素变化可能参与了柑橘第2次生理性落果。In order to explore the relation of endophytic fungi and fruit drop,the diversity of fungi in the dropping and healthy fruits of Newhall navel orange during the second fruit drop stage was studied by Illumina MiSeq sequencing technology.It was found that there were significant increases in the richness,evenness of the fungal community in the dropping fruits compared to the healthy fruits,and the composition structure was also different between them.Twenty-three endophytic fungal strains were isolated successfully from the dropping fruits and classified based on ITS sequences.Through artificial inoculation,some endophytic fungal strains could cause yellowing of the young citrus fruits and increase the content of abscisic acid and ethylene release in the fruits.The results suggest that the change of the endophytic fungal communities and the resulting change in the ABA and ETH might be involved in the second drop of citrus fruit.

关 键 词：柑橘 落果 内生真菌 真菌多样性 Illumina MiSeq 脱落酸 乙烯 

分 类 号：S666.4[农业科学—果树学]