水痘带状疱疹病毒gE蛋白联合不同佐剂对小鼠免疫效果的比较  

Comparison of immune effects of varicella zoster virus gE protein combined with different adjuvants in mice

作　　者：武婕慧 王瑞晨 付士红[2] 聂凯[2] 李樊 殷启凯 何英[2] 梁国栋[2] 王环宇[2] 李海 许松涛 Wu Jiehui;Wang Ruichen;Fu Shihong;Nie Kai;Li Fan;Yin Qikai;He Ying;Liang Guodong;Wang Huanyu;Li Hai;Xu Songtao(China Medical University,Shenyang 110000,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;WHO WPRO Regional Reference Measles/Rubella Laboratory,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]中国医科大学,沈阳110000 [2]中国疾病预防控制中心病毒病预防控制所,传染病溯源预警与智能决策全国重点实验室,北京102206 [3]中国疾病预防控制中心病毒病预防控制所,世界卫生组织西太平洋地区麻疹/风疹参比实验室,北京102206

出　　处：《中华实验和临床病毒学杂志》2023年第6期592-599,共8页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的比较利用AS01和Al/CpG联合佐剂,水痘带状疱疹病毒糖蛋白E(Varicella Zoster virus glycoprotein E,VZV gE)在BALB/c小鼠体内的免疫效果。方法BALB/c小鼠分别于0和21天免疫,小鼠血清抗体检测采用酶联免疫吸附实验;用VZV检测小鼠血清中和抗体滴度;酶联免疫吸附斑点实验检测细胞免疫反应。结果经过2次肌肉注射免疫,实验组(Shingrix、gE+Al/CpG和gE+AS01)中小鼠均能产生高滴度的中和抗体,增加分泌IFN-γ和IL-4的淋巴细胞数量。gE+Al/CpG免疫组中小鼠产生的中和抗体滴度最高(1943),但是AS01佐剂组(Shingrix和gE+AS01)中小鼠产生的分泌IFN-γ和IL-4的淋巴细胞数量高于Al/CpG佐剂组(gE+Al/CpG)。相比于AS01佐剂,Al/CpG佐剂诱导小鼠产生了偏向Th2型的体液免疫应答。各实验组中小鼠CD4+T细胞、CD8+T细胞的比例均没有统计学差异。结论Al/CpG佐剂联合gE蛋白诱导高滴度中和抗体,但是诱导产生的细胞免疫应答强度低于AS01佐剂组。Objective This study contrasts the immune efficacy of the varicella zoster virus glycoprotein E(VZV gE)using Al/CpG combined adjuvants and AS01 adjuvant in BALB/c mice.Methods BALB/c mice were immunized at 0 and 21 days respectively,and serum antibodies were detected using enzyme-linked immunosorbent assay.Detection of neutralizing antibodies in mouse serum using varicella zoster virus;enzyme-linked immunosorbent spot assay was used to detect cellular immune response.Results Following two intramuscular immunizations,mice in the experimental groups(Shingrix,gE+Al/CpG,and gE+AS01)demonstrated elevated neutralizing antibody titers and an augmented count of lymphocytes releasing IFN-γand IL-4.The gE+Al/CpG group displayed the highest neutralizing antibody titer(1943),yet the AS01-adjuvanted groups(Shingrix and gE+AS01)showed increased lymphocyte counts secreting IFN-γand IL-4 compared to the Al/CpG group(gE+Al/CpG).In comparison to the AS01 adjuvant,Al/CpG adjuvants triggered a humoral immune response favoring Th2 in mice.The proportions of CD4+T and CD8+T cells were not significantly different among the experimental groups.Conclusions Al/CpG adjuvant combined with gE protein resulted in high neutralizing antibody titers,while the intensity of the induced cellular immune response was inferior to that of AS01 adjuvant.

关 键 词：水痘带状疱疹病毒 糖蛋白E 佐剂 免疫效果 

分 类 号：R392[医药卫生—免疫学]