密蒙花标准汤剂UPLC指纹图谱建立及2种成分含量测定分析研究  

Establishment of UPLC fingerprint and quantitative analysis of two components of Buddlejae Flos standard decoction

作　　者：位翠杰 刘晓霞 段志文 冯涌微 张芳平 吴春秀 陈向东 孙冬梅 李振雨 Wei Cuijie;Liu Xiaoxia;Duan Zhiwen;Feng Yongwei;Zhang Fangping;Wu Chunxiu;Chen Xiangdong;Sun Dongmei;Li Zhenyu(Guangdong Yifang Pharmaceutical Co.,Ltd.,Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China;Longxi Yifang Pharmaceutical Co.,Ltd.,Dingxi 748100,China)

机构地区：[1]广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,佛山528244 [2]陇西一方制药有限公司,定西748100

出　　处：《国际中医中药杂志》2023年第12期1542-1548,共7页International Journal of Traditional Chinese Medicine

基　　金：国家工业和信息化部产业技术基础公共服务平台项目(2019-00902-1-2)。

摘　　要：目的建立密蒙花标准汤剂超高效液相色谱(UPLC)指纹图谱及有效成分毛蕊花糖苷和蒙花苷的含量测定方法。方法采用UPLC法建立17批密蒙花标准汤剂指纹图谱;利用相似度和聚类分析法对17批密蒙花标准汤剂进行评价分析;通过对照品对比对标准汤剂色谱峰进行归属;采用HPLC法测定密蒙花标准汤剂中有效成分毛蕊花糖苷和蒙花苷含量。结果密蒙花标准汤剂共呈现11个指纹峰,归属其中6个指纹峰;17批样品相似度为0.972~0.999,17批次标准汤剂聚类分为2类;采用指纹图谱方法对伪品结香花标准汤剂进行有效鉴别;含量测定结果显示,四川和湖北产地密蒙花饮片制备的标准汤剂中,毛蕊花糖苷和蒙花苷含量较高,且稳定。结论该方法可全面评价密蒙花标准汤剂质量,为其配方颗粒质量标准建立提供参考。Objective To establish UPLC fingerprint method of Buddlejae Flos standard decoction and determination method of acteoside and linarin.Methods UPLC method was used to establish the fingerprints of 17 batches of Buddlejae Flos standard decoction.Similarity evaluation and clustering analysis were carried out on the fingerprints of Buddlejae Flos standard decoction;the chromatographic peaks of standard decoction were identified by mass spectrometry and compared with the reference materials;the contents of acteoside and linarin in Buddlejae Flos standard decoction were determined by HPLC.Results There were 11 common peaks in the fingerprint of Buddlejae Flos standard decoction and 6 of them were identified.The similarity of the 17 batch samples was between 0.972 and 0.999.Clustering analysis classified 17 batches of Buddlejae Flos standard decoction into two categories;edgeworthia chrysantha standard decoction was identified by the method of fingerprint as counterfeit;the content determination results showed that the contents of acteoside and linarin in the standard decoction prepared from Buddlejae Flos of in Hubei and Sichuan Provinces were higher than others and were more stable.Conclusion The method can be used to comprehensively evaluate the quality of Buddlejae Flos standard decoction and provide reference for establishing the quality standard of Buddlejae Flos dispensing granules.

关 键 词：密蒙花 标准汤剂 指纹图谱 含量测定(中药) 结香花 

分 类 号：R284.1[医药卫生—中药学]