一个水稻基部叶角增大与早熟突变体blief的鉴定及基因定位  

Identifying and Gene Mapping of a Basal Leaves Inclination andEarly Flowering Mutant blief in Rice

作　　者：曾生元[1] 杜灿灿 胡庆峰 李闯[1] 景德道[1] 林添资[1] 孙立亭[1] 龚红兵[1] ZENG Shengyuan;DU Cancan;HU Qingfeng;LI Chuang;JING Dedao;LIN Tianzi;SUN Liting;GONG Hongbing(Zhenjiang Institute of Agricultural Sciences of the Ning-Zhen Hilly District,Jurong,Jiangsu 212400)

机构地区：[1]江苏丘陵地区镇江农业科学研究所,江苏句容212400

出　　处：《核农学报》2023年第12期2334-2341,共8页Journal of Nuclear Agricultural Sciences

基　　金：江苏省重点研发项目(BE2021360);镇江市重点研发项目(NY2022001)。

摘　　要：为了解析水稻株型与生育期的遗传调控网络,本研究以籼稻镇恢832辐射后代中得到的一个基部叶角增大、生育期提前的突变体(basal leaves inclination and early flowering,blief)为试验材料,对其进行表型鉴定、遗传分析、基因定位和候选基因分析。结果表明,与野生型相比,blief突变体出芽速度更快,基部前6张叶片角度显著变大、后恢复正常,在不同环境条件下抽穗期显著提前,株高等部分主要农艺性状发生了显著变化。遗传分析结果表明,blief受一对隐性核基因控制,采用图位克隆技术将BLIEF基因精细定位于第3染色体短臂插入缺失(InDel)标记BL3-24和BL3-27之间,物理距离为51 kb,基因测序、功能标记检验和定量表达分析结果判断BLIEF为光敏色素OsPhyB的一个功能缺失的等位基因。实时荧光定量PCR(qRT-PCR)分析结果表明,blief中多个生育期、株型调控相关基因的表达水平发生了显著变化。本研究结果为水稻株型及生育期的调控遗传网络研究和早熟高产育种实践提供了新的遗传资源。In order to elucidate the genetic network of plant architecture and flowering time in rice,a mutant named blief(basal leaves inclination and early flowering)which derived from irradiation of indica variety Zhenhui832(R832)was used as the material.Phenotype identification,genetic analysis,gene mapping and candidate gene analysis were performed.Results showed that:compared with the wild type,blief displayed stronger germinability,the angles of the basal six leaves were significantly increased,then recovered to erect.The heading date was significantly earlier than that of wild type under different environmental conditions,and main agronomic traits were changed significantly.Genetic analysis showed that blief was controlled by a recessive nuclear gene.BLIEF was fine mapped in a 51 kb interval between the InDel markers BL3-24 and BL3-27 on the short arm of Chromosome 3.Genomic sequencing,functional marker testing and qRT-PCR analysis deduced that BLIEF was a nonfunctional allele of Phytochrome B(OsPhyB).The expression level of several genes involved in photoperiodic response and plant architecture establishment were changed.The map-based cloning of BLIEF not only sheds a new light on understanding of the complex network of plant architecture establishment and flowering time in rice,but also provides genetic resource for high-yield,early-maturing rice variety breeding.

关 键 词：水稻 blief 株型 生育期 图位克隆 

分 类 号：S511[农业科学—作物学]