PGPR菌剂对攀西高原植烟土壤nifH基因群落的影响  

Effects of PGPR Inoculums on the nifH Gene Community in the Flue-Cured Tobacco Cultivation Soils of Panxi Plateau,China

作　　者：李思思 陈玉蓝 王勇 秦磊涛 陈强 辜运富 LI Sisi;CHEN Yulan;WANG Yong;QIN Leitao;CHEN Qiang;GU Yunfu(College of Resources,Sichuan Agricultural University,Chengdu,Sichuan 611130;Sichuan Tobacco Company Liangshan Prefecture Company,Xichang,Sichuan 615050)

机构地区：[1]四川农业大学资源学院,四川成都611130 [2]四川省烟草公司凉山州公司,四川西昌615050

出　　处：《核农学报》2023年第12期2496-2502,共7页Journal of Nuclear Agricultural Sciences

基　　金：四川省重点研发项目(2020YFN0100);四川省烟草公司凉山州公司项目(SCYC202104)。

摘　　要：为研究植物根际促生菌(PGPR)菌剂处理下攀西高原植烟土壤氮素循环的微生物调控机制,本研究以凉山州会理市不同PGPR菌剂(Lysinibacillus sp.、Streptomyces Phaeochromogenes和Aspergillus niger)处理下的植烟田为研究对象,采集烤烟成熟期根际土壤,通过化学分析和高通量测序技术对土壤理化性质、固氮酶活性及nifH基因群落组成及多样性进行分析。结果表明,与常规施肥相比,施用PGPR菌剂会显著降低植烟土壤的pH值,同时显著提高有效磷含量和固氮酶活性(P<0.05)。总体而言,施用PGPR菌剂提高了植烟土壤nifH基因群落的多样性。植烟土壤优势菌群为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和蓝菌门(Cyanobacteria)。慢生根瘤菌属(Bradyrhizobium)、地杆菌属(Geobacter)和固氮螺菌属(Azospirillum)等是植烟土壤优势属。冗余分析结果表明,植烟土壤pH值、碱解氮和速效钾含量与nifH基因群落结构相关性最强。综上,PGPR菌剂能改善植烟土壤养分状况,提高土壤固氮酶活性,影响植烟根际土壤nifH基因群落组成及多样性,其中以细菌PGPR菌剂Lysinibacillus sp.效果最佳。本研究结果为植烟土壤科学施肥、培肥地力提供了理论依据。We studied the nifH-gene communities and physico-chemical properties of rhizosphere soils from flue-cured tobacco amended with plant growth promoting Lysinibacillus sp.,Streptomyces phaeochromogenes and Aspergillus niger in Huili,Liangshan,China,strains to assess the connections between nitrogen cycling,fertilizer regimes and soil quality.In conventional fertilization,the application of plant growth promoting strains resulted in lower pH higher available phosphorus content and nitrogenase activity(P<0.05).Generally,the application of plant growth promoting strains increased the diversity of nifH-gene community.Proteobacteria,Firmicutes and Cyanobacteria were the dominant phyla,and Bradyrhizobium,Geobacter and Azospirillum were the dominant genera.In the redundancy analysis(RDA),soil pH and the contents of soil available potassium and available nitrogen were correlated with the nifH-gene containing bacterial community.In conclusion,the results suggested that a plant growth promoting strain inoculum could affect the nutritional status of flue-cured tobacco cultivation soil,increase the nitrogenase activity and modify the nifH-gene community composition and diversity.

关 键 词：植物根际促生菌(PGPR) NIFH基因 群落多样性 固氮酶活性 土壤理化性质 

分 类 号：S572[农业科学—烟草工业] S154[农业科学—作物学]