HIV假病毒模型优化及其在中医药防治艾滋病研究中的应用探讨  

Optimization of HIV Pseudovirus Model and Its Application in the Study of Chinese Medicine in the Prevention and Treatment of AIDS

作　　者：刘真[1] 邓博文[1] 李承乘 张清燕 桑锋[1] 王丹妮[1] 段晨晨 李强[1] LIU Zhen;DENG Bo-wen;LI Cheng-cheng;ZHANG Qing-yan;SANG Feng;WANG Dan-ni;DUAN Chen-chen;Li Qiang(Key Laboratory of Viral Disease Prevention and Treatment of TCM of Henan Province,Department of Acquired Immune Deficiency Syndrome Treatment and Research Center,the First Affiliated Hospital of Henan University of TCM,Zhengzhou,450000)

机构地区：[1]河南中医药大学第一附属医院艾滋病临床研究中心,河南省病毒性疾病中医药防治重点实验室,郑州450000

出　　处：《中国中西医结合杂志》2023年第12期1498-1501,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.82004207);河南省科技攻关计划(No.202102310178);河南省青年科学基金资助项目(No.202300410255);河南省中医管理局国家中医临床研究基地科研专项(No.2019JDZX2047,No.2021JDZY076,No.2021JDZY080,No.2018JDZX066);河南省中医药治疗艾滋病研究专项课题(No.2019AZB003);河南省病毒性疾病中医药防治重点实验室开放基金(No.SBDSYY2021-001,SBDSYY2021-002)。

摘　　要：中医药治疗艾滋病有一定疗效,但亟需研究其作用机制,因此建立稳定可靠模型尤为必要。HIV假病毒安全性高、稳定性强,筛选优势毒株构建假病毒感染细胞模型研究中药作用机制可行可信。假病毒模型已应用于体外药物筛选、中和抗体检测、表型耐药检验系统、宿主与病毒相互作用等研究方面。笔者在分析中药作用特点及中药研究手段的基础上,提出筛选优势毒株、优化病毒制备和感染过程可构建稳定可靠的假病毒感染细胞模型,并且可应用于中药多靶点的机制探讨及网络药理学活性中药筛选等,以期为更好地开展中医药防治艾滋病临床基础研究提供借鉴。Chinese medicine(CM)has a definite effect on acquired immune deficiency syndrome(AIDS),and it is urgent to study its mechanism.Therefore,it is particularly necessary to establish a stable and reliable model.HIV pseudoviruses are characterized by high safety and stability,it is feasible and credible to screen dominant strains to construct pseudovirus infection cell model and study the mechanism of CM.Pseudoviruses have been applied in drug screening,neutralizing antibody detection,phenotypic resistance testing system,host-virus interaction and so on.Based on the analysis of the characteristics and the research methods of CM,the authors proposed that a stable and reliable pseudovirus infection cell model can be constructed by screening dominant strains and optimizing the process of virus preparation and infection.The pseudovirus infection cell model can be used to explore the mechanism of multitarget of CM and network pharmacological activity of CM screening,so as to provide a reference for better clinical basic research of CM in prevention and treatment of AIDS.

关 键 词：人类免疫缺陷病毒假病毒 中医药 艾滋病 应用 

分 类 号：R259[医药卫生—中西医结合]