ZnFe_(2)O_(4)@聚多巴胺@Ag纳米复合材料的制备及其抑菌性  

Preparation and antibacterial properties of ZnFe_(2)O_(4)@polydopamine@Ag nanocomposites

作　　者：史娟 梁犇 宋凤敏 郝梦超 韩媛媛 吴迎花 邱莹 杨骏鹏 郭少波 SHI Juan;LIANG Ben;SONG Fengmin;HAO Mengchao;HAN Yuanyuan;WU Yinghua;QIU Ying;YANG Junpeng;GUO Shaobo(Shaanxi Key Laboratory of Catalysis,School of Chemistry and Environmental Sciences,Shaanxi University of Technology,Hanzhong 723001,China)

机构地区：[1]陕西理工大学化学与环境科学学院,陕西省催化基础与应用重点实验室,汉中723001

出　　处：《复合材料学报》2023年第12期6774-6788,共15页Acta Materiae Compositae Sinica

基　　金：陕西省科技厅项目(2020ZDLGY11-02,2021SF-382,2021JQ-752);陕西省科技厅项目(2023-JC-QN-0162);陕西理工大学秦巴生物资源与生态环境省部共建国家重点实验室项目(SXJ-2106);陕西理工大学科研一般项目(SLGKYXM2208)。

摘　　要：随着生活质量的提高,抗生素已成为人类不可或缺的药物,但近年来抗生素的滥用导致大量耐药菌出现对社会健康造成了严重的威胁。因此,迫切需要开发新型、有效持久的抗菌剂,以应对日益增长的公共卫生需求。本文先以FeCl_(3)、NaAc和ZnCl_(2)为原料用“热溶剂法”制备磁性铁酸锌(ZnFe_(2)O_(4)),再以ZnFe_(2)O_(4)为核进行聚多巴胺(PDA)包覆形成ZnFe_(2)O_(4)@PDA纳米微球,最后将由化学还原法制备的粒径在2~16 nm的银纳米颗粒(Ag NPs)负载于ZnFe_(2)O_(4)@PDA表面,形成ZnFe_(2)O_(4)@PDA@Ag纳米复合材料。通过TEM、XRD、XPS、UV-Vis、FTIR、Zeta电位等表征材料形貌特征。以革兰氏阴性菌铜绿假单胞菌(P.aeruginosa)、革兰氏阳性菌金黄色葡萄球菌(S.aureus)和耐药菌沙门氏菌(T-Salmonella)为模式菌,研究ZnFe_(2)O_(4)@PDA@Ag材料的抑菌活性及抑菌机制。实验结果表明,相比于同比例浓度的Ag NPs(负载量0.39%),材料对P.aeruginosa的抑菌率提升了57.1%、对S.aureus和T-Salmonella提升值分别为61.7%和39.2%。材料浓度为200μg/mL,作用时间60 min条件下,ZnFe_(2)O_(4)@PDA@Ag对测试菌抑制率均可达到99.9%。抑菌机制结果证实,ZnFe_(2)O_(4)@PDA@Ag可与细胞壁表面蛋白作用破坏细胞壁,进入细菌内部与胞内蛋白和相关酶作用阻碍细胞呼吸,且破坏DNA结构并抑制其复制过程,从而影响细菌呼吸和细胞分裂等生理生化过程,最终导致细菌死亡。该材料以磁性ZnFe_(2)O_(4)为内核,具有可重复利用、高性价比、无二次污染等优点;PDA层包覆使材料具有良好的生物相容性。同时,Ag NPs在ZnFe_(2)O_(4)@PDA纳米微球表面的负载,解决了Ag NPs易团聚问题,且因小颗粒Ag NPs可直接通过离子通道进入细菌内部,使ZnFe_(2)O_(4)@PDA@Ag具备了优异的抗菌活性。本工作可为新型、智能化抗生素材料的研发提供理论依据。With the improvement of life quality,antibiotics have become indispensable drugs for human beings.However,the prevalence of multiple super pathogenic bacteria in environments are induced by the extensive use of antibiotics,which poses a serious threat to social health.It has become extremely urgent to develop new,effective and durable antibacterial agents in response to a rising publichealth demand.In this paper,magnetic zinc ferrite(ZnFe_(2)O_(4))was prepared by one-pot method using FeCl_(3),NaAc and ZnCl_(2) as raw materials.Secondly,ZnFe_(2)O_(4)@PDA nanospheres were formed by coating polydopamine(PDA)on the surface of ZnFe_(2)O_(4).Finally,silver nanoparticles(Ag NPs)with particle size of 2-16 nm prepared by chemical reduction method were adsorbed on the surface of ZnFe_(2)O_(4)@PDA nanospheres to form ZnFe_(2)O_(4)@PDA@Ag nanocomposites.The prepared nanocomposite was characterized by TEM,XRD,XPS,UV-Vis,FTIR and Zeta potential.The antimicrobial activity and mechanism of Zn-Fe_(2)O_(4)@PDA@Ag were studied with gram-negative bacteria P.eruginosa,gram-positive bacteria S.aureus and drugresistant bacteria T-Salmonella.Compared with the same concentration of Ag NPs(loading 0.39%),the antibacterial rate of the material against P.aeruginosa was increased by 57.1%,and that against S.aureus and T-Salmonella was increased by 61.7%and 39.2%,respectively.When tested bacterial were treated for 60 min in 200μg/mL ZnFe_(2)O_(4)@PDA@Ag,the inhibition rates of the material to the three test bacteria reached 99.9%.The results of bacteriostasis mechanism showed that ZnFe_(2)O_(4)@PDA@Ag could interact with cell wall surface proteins to destroy cell wall,enter the interior of bacterial and interact with intracellular proteins and related enzymes to hinder cell respiration,damage DNA structure,and inhibit its replication process,thus affecting physiological and biochemical processes such as bacterial respiration and cell division,and eventually lead to bacterial death.With magnetic ZnFe_(2)O_(4) as the core,the nanocomposite is

关 键 词：ZnFe_(2)O_(4)@聚多巴胺(PDA)@Ag 纳米材料 抑菌机制 抑菌材料 Ag纳米颗粒(NPs) 

分 类 号：TB333[一般工业技术—材料科学与工程] O614[理学—无机化学]